中文摘要：水稻条纹病毒（RSV） 是水稻种严重的植物病原病毒之一，由灰飞虱传播。RNA干扰（RNAi）是真核生物（包括昆虫）中抗病毒感染的一种先天防御机制。为了干扰灰飞虱体内RSV利用RNAi途径进行的传播，通过灌溉水稻叶片间接将RSV衍生的dsRNAs导入昆虫体内。结果，导入RSV衍生dsRNAs的灰飞虱体内RSV复制以计量依赖性方式减少，这表明这些dsRNA可能会诱导灰飞虱体内先天抗病毒RNAi途径，并因此抑制RSV的复制。
外文摘要：Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice and transmitted by small brown planthopper, Laodelphax striatellus. RNA interference (RNAi) is an innate defense mechanism against viral infection in eukaryotic organisms including insects. To disturb transmission of the RSV in L. striatellus using RNAi pathway, RSV-derived dsRNAs were applied to the insects indirectly through rice leaves by irrigation. As a result, replication of RSV in L. striatellus ingested RSV-derived dsRNAs was reduced in dose-dependent manner, suggesting that these dsRNA could induce innate antiviral RNAi pathway in L. striatellus and therefore suppress replication of RSV.
外文关键词：Rice stripe virus; Laodelphax striatellus; RNA interference; Double-stranded RNA
作者：An, Saes Byeol; Choi, Jae Young; Lee, Seok Hee; 等
作者单位：韩国首尔国立大学
期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
期刊影响因子：1.046
出版年份：2017
出版刊次：6
点击下载：通过病毒双链RNAs使灰飞虱体内水稻条纹病毒沉默的研究