中文摘要：Remorin是植物特异性膜相关蛋白，在植物-病原体相互作用中发挥关键作用。然而，对于病原体如何抵抗重组蛋白介导的寄主反应知之甚少。本研究通过定量全蛋白质组分析发现remorin蛋白（NbREM1）在水稻条纹病毒（RSV）侵染初期是下调的。进一步研究发现NbREM1的流动受S-酰化修饰调节，其降解主要通过自噬途径介导。RSV可以干扰NbREM1的S-酰化。本研究相关研究结果表明，通过干扰remorin 蛋白S-酰化下调蛋白水平是RSV克服remorin介导的抑制病毒移动的常用策略。
外文摘要：Remorins are plant-specific membrane-associated proteins and were proposed to play crucial roles in plant-pathogen interactions. However, little is known about how pathogens counter remorin-mediated host responses. In this study, by quantitative whole-proteome analysis we found that the remorin protein (NbREM1) is downregulated early in Rice stripe virus (RSV) infection. We further discovered that the turnover of NbREM1 is regulated by S-acylation modification and its degradation is mediated mainly through the autophagy pathway. Interestingly, RSV can interfere with the S-acylation of NbREM1, which is required to negatively regulate RSV infection by restricting virus cell-to-cell trafficking. The disruption of NbREM1 S-acylation affects its targeting to the plasma membrane microdomain, and the resulting accumulation of non-targeted NbREM1 is subjected to autophagic degradation, causing downregulation of NbREM1. Moreover, we found that RSV-encoded movement protein, NSvc4, alone can interfere with NbREM1 S-acylation through binding with the C-terminal domain of NbREM1 the S-acylation of OsREM1.4, the homologous remorin of NbREM1, and thus remorin-mediated defense against RSV in rice, the original host of RSV, indicating that downregulation of the remorin protein level by interfering with its S-acylation is a common strategy adopted by RSV to overcome remorin-mediated inhibition of virus movement.
外文关键词：remorins; S-acylation; autophagy; rice stripe virus; movement
作者：Fu, Shuai; Xu, Yi; Li, Chenyang; 等
作者单位：浙江大学
期刊名称：MOLECULAR PLANT
期刊影响因子：8.827
出版年份：2018
出版刊次：2
点击下载：水稻条纹病毒干扰Remorin S-酰化并诱导其自噬性降解以促进病毒感染的研究