中文摘要：水稻条纹病毒p2可能会通过与烟草全长纤维蛋白（NbFib2）在细胞核和卡侯氏体(CB)中的交互作用从而促进病毒系统性感染。NbFib2含有3个功能域。本文采用酵母双杂交、共定位和双分子荧光互补（BiFC）分析技术研究了p2和NbFib2的3个结构域（即含甘氨酸和精氨酸（GAR）结构域的N-末端片段，中心RNA结合结构域和含有α-螺旋结构域的C-末端片段）之间的交互作用。结果显示，N端结构域是NbFib2在核仁和卡侯氏体中的定位所必不可少的。p2可与NbFib2的3个结构域相结合，并以细胞核为靶标，但p2不能与细胞核和卡侯氏体(CBs)相结合。
外文摘要：p2 of Rice stripe virus may promote virus systemic infection by interacting with the full length of fibrillarin from Nicotiana benthamiana (NbFib2) in the nucleolus and cajal body (CB). NbFib2 contains three functional domains. We used yeast two-hybrid, colocalization, and bimolecular fluorescence complementation (BiFC) assays to study the interactions between p2 and the three domains of NbFib2, namely, the N-terminal fragment containing a glycine and arginine-rich (GAR) domain, the central RNA-binding domain, and the C-terminal fragment containing an alpha-helical domain. The results show that the N-terminal domain is indispensable for NbFib2 to localize in the nucleolus and cajal body. p2 binds all three regions of NbFib2, and they target to the nucleus but fail to the nucleolus and cajal bodies (CBs).
作者：Zheng, Luping; He, Jie; Ding, Zuomei; 等
作者单位：福建农林大学
期刊名称：CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
期刊影响因子：1.459
出版年份：2018
出版刊次：3
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