中文摘要：植物病毒可通过表达能增强致病性的抑制子蛋白来抑制寄主抗病毒RNA沉默。番茄褪绿病毒（ToCV）基因组编码RNA沉默抑制子p22蛋白（进行烟草分析时发现p22蛋白局部抑制子活性持续时间最长）。由于RNA沉默抑制与其增强病害严重程度的能力密切相关，作者研究分析了p22表达在异源病毒环境中的影响。因此，作者研究了来自病毒介体烟草脆裂病毒（TRV）和马铃薯X病毒（PVX）的以及来自于烟草衰退抑制子变体的ToCV p22表达的影响。研究结果表明：虽然在两种病毒ToCV p22异源表达中观察到导致植物死亡的病害症状有加重趋势，但只在TRV上发生病毒累积增加的现象。ToCV p22的异源表达不能补偿抑制子缺损的突变体病毒。
外文摘要：To counteract host antiviral RNA silencing, plant viruses express suppressor proteins that function as pathogenicity enhancers. The genome of the Tomato chlorosis virus (ToCV) (genus Crinivirus, family Closteroviridae) encodes an RNA silencing suppressor, the protein p22, that has been described as having one of the longest lasting local suppressor activities when assayed in Nicotiana benthamiana. Since suppression of RNA silencing and the ability to enhance disease severity are closely associated, we analyzed the effect of expressing p22 in heterologous viral contexts. Thus, we studied the effect of the expression of ToCV p22 from viral vectors Tobacco rattle virus (TRV) and Potato virus X (PVX), and from attenuated suppressor mutants in N. benthamiana plants. Our results show that although an exacerbation of disease symptoms leading to plant death was observed in the heterologous expression of ToCV p22 from both viruses, only in the case of TRV did increased viral accumulation occur. The heterologous expression of ToCV p22 could not complement suppressor-defective mutant viruses.
外文关键词：Closteroviridae; Crinivirus; RNA silencing suppressor; Tomato chlorosis virus; virus pathogenicity
作者：Yazmín Landeo-Ríos; Jesús Navas-Castillo; Enrique Moriones; M. Carmen Cañizares
作者单位：马拉加大学
期刊名称：Viruses
期刊影响因子：3.465
出版年份：2017
出版刊次：11
点击下载：来自烟草脆裂病毒和马铃薯X病毒的番茄褪绿病毒p22 RNA沉默抑制子的异源表达增强病害严重性，但不补偿抑制子-缺损的突变体病毒