中文摘要：菜豆金色花叶病毒属病毒是仅由烟粉虱以持久、循环方式传播的病毒，可对全球作物生产造成重大的经济损失。先前研究已表明，菜豆金色花叶病毒属病毒在烟粉虱中肠细胞囊泡状结构中累积，网格蛋白介导的内吞作用负责其内化。然而，菜豆金色花叶病毒属病毒在烟粉虱中肠细胞中的运输过程大多仍是未知。本文研究了囊泡运输在番茄黄化曲叶病毒（TYLCV）运输中的作用。研究结果表明了早期内体和管状内体网络在菜豆金色花叶病毒属病毒运输中的重要性。
外文摘要：Begomoviruses are exclusively transmitted by whiteflies in a persistent circulative manner and cause considerable economic losses to crop production worldwide. Previous studies have shown that begomoviruses accumulate in vesicle-like structures in whitefly midgut cells and that clathrin-mediated endocytosis is responsible for their internalization. However, the process by which begomoviruses are trafficked within whitefly midgut cells remains largely unknown. In this study, we investigated the roles of vesicle trafficking in the transport of Tomato yellow leaf curl virus (TYLCV), a begomovirus that has spread to over 50 countries and caused extensive damage to a range of important crops, within midgut cells of whitefly (Bemisia tabaci). By disrupting vesicle trafficking using RNA silencing and inhibitors, we demonstrated that the early steps of endosomal trafficking are important for the intracellular transport of TYLCV in the whitefly midgut. In addition, our data show that, unlike many animal viruses, TYCLV is trafficked within cells in a manner independent of recycling endosomes, late endosomes, lysosomes, the Golgi apparatus and the endoplasmic reticulum. Instead, our results suggest that TYLCV might be transported directly from early endosomes to the basal plasma membrane and released into the hemolymph. Silencing of the sorting nexin Snx12, which may be involved in membrane tubulation, resulted in fewer viral particles in hemolymph; this suggests that the tubular endosomal network may be involved in the transport of TYLCV. Our results also support a role for the endo-lysosomal system in viral degradation. We further showed that the functions of vector early endosomes and sorting nexin Snx12 are conserved in the transmission of several other begomoviruses. Overall, our data indicate the importance of early endosomes and the tubular endosomal network in begomovirus transmission.
作者：Xia WQ;Liang Y;Chi Y;Pan LL;Zhao J;Liu SS;Wang XW
作者单位：浙江大学
期刊名称：PLoS Pathog
期刊影响因子：6.608
出版年份：2018
出版刊次：1
点击下载：菜豆金色花叶病毒属病毒在昆虫介体中肠细胞内的运输研究