中文摘要：植食性半翅目昆虫传播植物病毒涵盖了复杂的交互作用（在病毒采集、保留和接种的连续过程中）。作者研究调查了饲料PH值对烟粉虱取食行为和 莴苣留存在介体前肠中的传染性枯黄病毒 (LIYV)病毒粒子释放的影响。电渗透图分析显示，在不同PH值（4，7.4和9）的人工饲料中，与烟粉虱探查与取食相关的变量没有明显差异。研究结果表明，人工饲料中仅PH值发生变化并不会导致烟粉虱取食行为发生明显改变；人工饲料PH值和前肠病毒体的释放/接种之间存在着一种潜在的因果和可能关系。
外文摘要：Transmission of plant viruses by phytophagous hemipteran insects encompasses complex interactions underlying a continuum of processes involved in virus acquisition, retention and inoculation combined with vector feeding behavior. Here, we investigated the effects of dietary pH on whitefly (Bemisia tabaci) feeding behavior and release of Lettuce infectious yellows virus (LIYV) virions retained in the vector's foregut. Electrical penetration graph analysis revealed that variables associated with whitefly probing and ingestion did not differ significantly in pH (4, 7.4, and 9) adjusted artificial diets. To investigate virus retention and release, whiteflies allowed to acquire LIYV virions in a pH 7.4 artificial diet were fed pH 4, 7.4, or 9 virion-free artificial (clearing) diets. Immunofluorescent localization analyses indicated that virions remained bound to the foreguts of approximately 20%-24% of vectors after they fed on each of the 3 pH-adjusted clearing diets. When RNA preparations from the clearing diets were analyzed by reverse transcription (RT) nested-PCR and, in some cases, real-time qPCR, successful amplification of LIYV-specific sequence was infrequent but consistently repeatable for the pH 7.4 diet but never observed for the pH 4 and 9 diets, suggesting a weak pH-dependent effect for virion release. Viruliferous vectors that fed on each of the 3 pH-adjusted clearing diets transmitted LIYV to virus-free plants. These results suggest that changes in pH values alone in artificial diet do not result in observable changes in whitefly feeding behaviors, an observation that marks a first in the feeding of artificial diet by whitefly vectors; and that there is a potential causal and contingent relationship between the pH in artificial diet and the release/inoculation of foregut bound virions.
外文关键词：Crinivirus; dietary pH; inoculation; noncirculative semipersistent transmission; retention; whitefly
作者：Zhou, Jaclyn S.; Chen, Angel Y. S.; Drucker, Martin; 等.
作者单位：加州大学系统
期刊名称：INSECT SCIENCE
期刊影响因子：2.026
出版年份：2017
出版刊次：12
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