中文摘要：MicroRNAs (miRNAs) 是一种内源性非编码小RNA，在植物发育和环境胁迫反应中起着重要的调控作用。玉米是全球重要的经济性粮食和饲料作物。迄今为止，已经鉴定出许多玉米miRNAs参与了植物发育和胁迫反应。然而，对于玉米中响应病毒入侵的由miRNA介导的基因调控网络大多仍然是未知。本研究通过高通量测序分别获得缓冲区和接种甘蔗花叶病毒（SCMV）的玉米中的小RNA的图谱。共检测到154种已知miRNA和213种新型miRNA，并且大多数miRNA在SCMV感染后差异表达。另外，通过降解组分析鉴定了13个已知miRNA的70个靶标和1个新miRNA的3个靶标。诺森印迹法和实时定量PCR结果显示，所选miRNAs及其靶标的表达水平主要受接种12天后SCMV感染的影响，包括miR168和miR528的上调以及miR159、miR397和miR827的下调。这些结果为研究玉米中miRNA的调控网络及其响应SCMV侵染的靶标提供了新视角。
外文摘要：MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses. Maize (Zea mays L.) is a global economically important food and forage crop. To date, a number of maize miRNAs have been identified as being involved in plant development and stress responses. However, the miRNA-mediated gene regulatory networks responsive to virus infections in maize remain largely unknown. In this study, the profiles of small RNAs in buffer- and Sugarcane mosaic virus (SCMV)-inoculated maize plants were obtained by high-throughput sequencing, respectively. A total of 154 known miRNAs and 213 novel miRNAs were profiled and most of the miRNAs identified were differentially expressed after SCMV infection. In addition, 70 targets of 13 known miRNAs and 3 targets of a novel miRNA were identified by degradome analysis. The results of Northern blotting and quantitative real-time PCR showed that the expression levels of the selected miRNAs and their targets were mostly influenced by SCMV infection at 12 days post inoculation, including up-regulation of miR168 and miR528, and down-regulation of miR159, miR397 and miR827. These results provide new insights into the regulatory networks of miRNAs and their targets in maize plants responsive to SCMV infection.
外文关键词：Sugarcane mosaic virus (SCMV); Maize; MiRNAs; High-throughput sequencing; Degradome analysis
作者：Xia, Zihao; Zhao, Zhenxing; Li, Mingjun; 等
作者单位：中国农业大学
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY
期刊影响因子：2.724
出版年份：2018
出版刊次：4
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