中文摘要：帕兰波番茄曲叶病毒（ToLCPalV）是一种烟粉虱传播的双生菜豆金黄花叶病毒属病毒。本文研究证实了来自马铃薯X病毒（PVX）介体AV2的异常表达加速了烟草的系统性坏死和活性氧（ROS）的积累。此外，研究发现AV2的N-末端区域10个氨基酸与系统性坏死症状/表型相关。在酵母双杂交测定中发现Catalase2 (Cat2)与AV2蛋白相结合，且Cat2的沉默导致烟草上出现坏死病变，并且与对照植物相比，这些植物对ToLCPalV感染高度敏感。研究结果表明，AV2对于寄主中的致病性、系统性运动和基因沉默的抑制是必需的。总之，本研究结果表明，AV2和Cat2的交互作用可能在ToLCPalV成功侵染中起着关键作用。
外文摘要：Tomato leaf curl Palampur virus (ToLCPalV) is a whitefly-transmitted, bipartite begomovirus. Here, we demonstrated that ectopic expression of AV2 from a Potato virus X (PVX)-based vector accelerated systemic necrosis and reactive oxygen species (ROS) accumulation in Nicotiana benthamiana. Furthermore, 10 amino acids from N-terminal region of AV2 were found to be associated with the systemic necrosis symptom/phenotype. Mutational studies of ToLCPalV infectious clones lacking the AV2 revealed that AV2 is essential for the systemic movement of DNA-A, symptom severity and viral DNA accumulation. In a yeast two-hybrid assay, Catalase2 (Cat2) was found to associate with AV2 protein. Further, silencing of Cat2 resulted in appearance of necrotic lesions on N. benthamiana and these plants were highly susceptible to ToLCPalV infection in comparison to control plants. Infection ToLCPalV on Solanum lycopersicum resulted in downregulation of Cat2 transcripts, followed by accumulation of ROS and stress marker transcripts. The AV2 protein also suppressed virus-induced gene silencing (VIGS) of the Phytoene desaturase (PDS) gene. Our results show that AV2 is essential for the pathogenicity, systemic movement and suppression of gene silencing in the host. Altogether, our findings suggest that interactions between AV2 and Cat2 might play a crucial role in the establishment of ToLCPalV infection.
作者：Roshan, Poonam; Kulshreshtha, Aditya; Kumar, Surender; 等.
作者单位：Acad Sci & Innovat Res AcSIR
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2018
出版刊次：1
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