中文摘要：Drosophila E75属于核受体超家族，在包括蜕皮激素信号在内的各种生理过程中起着关键作用。 本文研究鉴定了灰飞虱Drosophila E75（LsE75）的一种直系同源基因。系统发育分析表明，LsE75 聚集在昆虫E75组内，并且与半翅目昆虫的E75密切相关。定量PCR分析显示，RSV带毒灰飞虱体内LsE75的转录比非带毒灰飞虱体内高。为了抑制RSV在灰飞虱体内利用RNAi途径进行传播，对昆虫外源饲喂针对LsE75 (dsLsE75)的dsRNA。研究结果表明，取食dsLsE75的灰飞虱体内RSV NS3基因的转录以计量依赖性方式降低，这说明沉默RSV带毒灰飞虱体内LsE75转录可抑制RSV的传播。
外文摘要：Drosophila E75 belongs to the nuclear receptor superfamily and plays key role in various physiological processes including ecdysone signaling. We identified an orthologue of Drosophila E75 from the Laodelphax striatellus (LsE75). Open reading frame (ORF) of the LsE75 contains 2454 nucleotides and encodes a protein of 817 amino acids. A phylogenetic analysis showed that the LsE75 clustered within the insect E75 group and was most closely related to the E75 from hemipteran insects. Quantitative PCR analysis revealed that transcription of the LsE75 was highly increased in RSV-viruliferous L. striatellus compared to that in non-viruliferous L. striatellus. To inhibit transmission of the RSV in L. striatellus using RNAi pathway, dsRNA against LsE75 (dsLsE75) was exogenously applied to the insects. As a result, transcription of RSV NS3 gene in L. striatellus ingested dsLsE75 was reduced in dose-dependent manner, suggesting that transmission of the RSV could be suppressed by silencing of LsE75 transcription in RSV-viruliferous L. striatellus.
外文关键词：Nuclear receptor E75; Laodelphax striatellus; Rice stripe virus; RNA interference; Double-stranded RNA
作者：Fang, Ying; Choi, Jae Young; Lee, Seok Hee; 等
作者单位：首尔大学
期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
期刊影响因子：1.046
出版年份：2017
出版刊次：12
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