中文摘要：农作物中的植物病毒在世界范围内受到极大的关注，其中超过75%的病毒通过昆虫介体从感染病毒的植物传播到健康植株上。番茄黄化曲叶病毒（TYLCV）是一种由烟粉虱传播的植物病毒中最大且经济上最重要的菜豆金黄花叶病毒属病毒。TYLCV在昆虫中的循环包括复杂的昆虫-病毒互作，而这些互作的分子机制仍不明确。昆虫肠道作为病毒进入和传播的屏障，被认为可调节介体的特异性。然而，由于粉虱肠道体积较小，关于其对病毒侵染的响应方面的信息有限。本研究采用Illumina测序法研究了B型烟粉虱EAM1小种肠道对TYLCV侵染的转录响应。研究结果揭示了粉虱肠道中复杂的昆虫-病毒关系，并为昆虫中肠在病毒传播中的作用提供了大量的分子信息。
外文摘要：Background: Plant viruses in agricultural crops are of great concern worldwide, and over 75% of them are transmitted from infected to healthy plants by insect vectors. Tomato yellow leaf curl virus (TYLCV) is a begomovirus, which is the largest and most economically important group of plant viruses, transmitted by the whitefly Bemisia tabaci. The circulation of TYLCV in the insect involves complex insect-virus interactions, whereas the molecular mechanisms of these interactions remain ambiguous. The insect gut as a barrier for viral entry and dissemination is thought to regulate the vector specificity. However, due to its tiny size, information for the responses of whitefly gut to virus infection is limited. Methods: We investigated the transcriptional response of the gut of B. tabaci Middle East-Asia Minor 1 species to TYLCV infection using Illumina sequencing. Results: A total of 5207 differentially expressed genes (DEGs) between viruliferous and non-viruliferous whitefly guts were identified. Enrichment analyses showed that cargo receptor and ATP-binding cassette (ABC) transporters were enriched in DEGs, and might help the virus to cross gut barrier. TYLCV could perturb cell cycle and DNA repair as a possible result of its replication in the whitefly. Our data also demonstrated that TYLCV can activate whitefly defense responses, such as antimicrobial peptides. Meanwhile, a number of genes involved in intracellular signaling were activated by TYLCV infection.Conclusions: Our results reveal the complex insect-virus relationship in whitefly gut and provide substantial molecular information for the role of insect midguts in virus transmission.
外文关键词：Transcriptome; Whitefly; TYLCV; Gut; Gene expression
作者：Geng, Liang; Qian, Li-Xin; Shao, Ruo-Xuan; 等
作者单位：浙江大学
期刊名称：VIROLOGY JOURNAL
期刊影响因子：2.139
出版年份：2018
出版刊次：1
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