中文摘要：从几个温室采集粉虱样品，并通过聚合酶链式反应（PCR）鉴定为烟粉虱，接着测定mtCOI基因序列显示其为B型MEAM1。本研究通过开展几个实验鉴定甜瓜褪绿黄化病毒（CCYV）是由所鉴定的B型烟粉虱传播的。实验结果表明，单个烟粉虱足以将病毒传播给健康幼苗，还发现介体需要90分钟的采集与接种才能成果转移病毒。此外，发现介体在获得病毒后可保留病毒6天。通过对比研究结果表明，与黄瓜相比，曼陀罗是一种相对较低效的粉虱获得性病毒来源。CCYV的寄主范围实验表明，所有葫芦科植物都被感染并且具有不同程度的症状。黄瓜、甜瓜、苋色藜和曼陀罗显示出脉间黄化，而在所有实证检验的宿主中均观察到黄化和生长发育减慢。
外文摘要：Whitefly samples were collected from several greenhouses and identified as Bemisia tabaci by polymerase chain reaction (PCR), followed by sequencing of mtCOI gene that revealed their identity as biotype B MEAM1. Transmission of Cucurbit chlorotic yellows virus (CCYV) by the identified whitefly biotype was estimated by conducting several experiments. The results showed that a single whitefly was sufficient to transmit the virus to healthy seedlings. It was also found that the vector required a 90-min acquisition and inoculation access period to successfully transfer the virus. Moreover, vector was found to be able to retain the virus for 6 days' post acquisition. Efficiency of cucumber and D. stramonium, plants was compared as a source of CCYV inoculum for virus acquisition by B. tabaci and obtained results revealed that cucumber was a more efficient source of virus infection than D. stramonium. Using cucumber as source of infection, 90% of the inoculated plants (from each species i.e. cucumber and D. stramonium), were infected while using D. stramonium, as a source, the percentage of infection in cucumber plants dropped to 60% (cucumber) and to 50% in D. stramonium. These results showed that D. stramonium, is a relatively less efficient source of virus for whitefly acquisition as compared to cucumber. Host range experiments for CCYV showed that all the cucurbit plants were infected and had different levels of symptoms. Cucumis sativus, C. melo, C. amaranticolor and D. stramonium showed interveinal chlorosis, whereas general yellowing and reduced growth were observed in all positively tested hosts.
外文关键词：Bemisia tabaci; Biotype B; CCYV; Host range; Transmission
作者：Shakeel, Muhammad Taimoor; Al-Saleh, Mohammad Ali; Amer, Mahmoud Ahmed; 等.
作者单位：沙特阿拉伯国王大学
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
期刊影响因子：0.746
出版年份：2018
出版刊次：2
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