中文摘要：由菜豆金黄花叶病毒属编码的V2蛋白对于病毒感染是必不可少的，并且涉及到多种功能，如病毒移动和宿主防御反应的抑制。本文研究报道了由TYLCV（番茄上感染最严重的病毒之一）编码的V2可以与其自身相互作用，而V2的S71A突变 V2(S71A) 取消了其资自身交互作用。研究表明，71位点上的氨基酸也涉及到V2的致病性。本研究首次指出由TYLCV编码的V2的S71A突变会影响V2的自我相互作用、聚集物的形成和致病性。
外文摘要：The V2 protein encoded by Begomovirus is essential for virus infection and is involved in multiple functions, such as virus movement and suppression of the host defence response. In this study, we reported that V2 encoded by the Tomato yellow leaf curl virus (TYLCV), which is one of the most devastating tomato-infecting begomoviruses, could interact with itself and a S71A mutation of V2 (V2(S71A)) abolished its self-interaction. Fluorescence results showed that V2 localized primarily in the cytoplasm and around the nucleus. Site-directed mutagenesis V2(S71A) had the similar subcellular localization, but V2(S71A) formed fewer large aggregates in the cytoplasm compared to wild-type V2, whereas the level of aggregates came to a similar after treatment with MG132, which indicates that the S71A mutation might affect 26S proteasome-mediated degradation of V2 aggregates. Meanwhile, heterologous expression of V2(S71A) from a Potato virus X vector induced mild symptoms compared to wild-type V2, delay of virus infection associated with mild symptoms was observed in plants inoculated with TYLCV-S71A, which indicates that the amino acid on position 71 is also involved in the pathogenicity of V2. To the best of our knowledge, this report is the first to state that the S71A mutation of V2 encoded by TYLCV affects the self-interaction, aggregate formation and pathogenicity of V2.
作者：Zhao, Wenhao; Ji, Yinghua; Wu, Shuhua; 等
作者单位：南京农业大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2018
出版刊次：2
点击下载：由TYLCV编码的V2中的单个氨基酸对自身交互作用、聚集性及致病性的影响研究