中文摘要：Armadillo重复家系在多种植物物种中具有很好的特征表达，它们参与了多种调控过程，包括生长、发育和胁迫反应。之前研究已表明，在病毒侵染时，ARM蛋白编码在ToLCNDV耐受番茄品种中的表达比易感番茄品种中高出3倍之多。这表明ARM蛋白在抗病毒感染防御反应中可能有所参与，但并没有对此进行全面调查。本研究鉴定了番茄中的46个ARM-重复蛋白（SlARMs）和41个含U-box的蛋白（SlPUBs），并研究了这些蛋白质及其相应基因的上游启动子区域的理化性质、基因结构、结构域、染色体位点、系统发育和顺式作用元件。研究结果表明，SlARM18基因参与了ToLCNDV的抗性机制。
外文摘要：Armadillo repeat family is well-characterized in several plant species for their involvement in multiple regulatory processes including growth, development, and stress response. We have previously shown a three-fold higher expression of ARM protein-encoding in tomato cultivar tolerant to tomato leaf curl New Delhi virus (ToLCNDV) compared to susceptible cultivar upon virus infection. This suggests the putative involvement of ARM proteins in defense response against virus infection; however, no comprehensive investigation has been performed to address this inference. In the present study, we have identified a total of 46 ARM-repeat proteins (SlARMs), and 41 U-box-containing proteins (SlPUBs) in tomato. These proteins and their corresponding genes were studied for their physicochemical properties, gene structure, domain architecture, chromosomal localization, phylogeny, and cis-regulatory elements in the upstream promoter region. Expression profiling of candidate genes in response to ToLCNDV infection in contrasting tomato cultivars showed significant upregulation of SlARM18 in the tolerant cultivar. Virus-induced gene silencing of SlARM18 in the tolerant tomato cultivar conferred susceptibility, which suggests the involvement of this gene in resistance mechanism. Further studies are underway to functionally characterize SlARM18 to delineate its precise role in defense mechanism.
外文关键词：Tomato (Solanum lycopersicum); Tomato leaf curl New Delhi virus (ToLCNDV); Armadillo repeat family; U-box domain; Virus-induced gene silencing
作者：Mandal, Arunava; Mishra, Awdhesh Kumar; Dulani, Priya; 等
作者单位：National Institute of Plant Genome Research (NIPGR)
期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS
期刊影响因子：3.496
出版年份：2018
出版刊次：3
点击下载：番茄中ARC蛋白的鉴定、特征、表达谱和病毒诱导的基因沉默表明其参与了新德里番茄曲叶病毒的抗性