中文摘要：昆虫卵黄蛋白原（Vg）被认为是在脂肪体内合成的。本文作者研究发现，在灰飞虱血细胞中也合成了丰富的Vg蛋白。研究还确定了只有血细胞产生的Vg蛋白与体内水稻条纹病毒（RSV）相结合。对灰飞虱Vg（LsVg）亚基组成的检测表明，LsVg在不同组织中表达后会经历不同的处理。能与RSV结合的LsVg亚基仅稳定存在于血细胞中，而脂肪体产生的LsVg缺乏RSV相互作用亚基。灰飞虱若虫和雌虫也合成Vg，但仅在血细胞中合成，且Vg蛋白质与RSV共同集中在一起。此外，还观察到RNA干扰敲除LsVg转录物降低了血淋巴中的RSV滴度，从而干扰了病毒系统性侵染。该项研究结果揭示了LsVg的性别依赖性表达和组织特异性加工，并且前所未有地将该蛋白质在介导病毒传播中的功能与其组织中存在的先前认为非Vg产生的特定分子形式联系起来。
外文摘要：Insect vitellogenin (Vg) has been considered to be synthesized in the fat body. Here, we found that abundant Vg protein is synthesized in Laodelphax striatellus hemocytes as well. We also determined that only the hemocyte-produced Vg binds to Rice stripe virus (RSV) in vivo. Examination of the subunit composition of L. striatellus Vg (LsVg) revealed that LsVg was processed differently after its expression in different tissues. The LsVg subunit able to bind to RSV exist stably only in hemocytes, while fat body-produced LsVg lacks the RSV-interacting subunit. Nymph and male L. striatellus individuals also synthesize Vg but only in hemocytes, and the proteins co-localize with RSV. We observed that knockdown of LsVg transcripts by RNA interference decreased the RSV titer in the hemolymph, and thus interfered with systemic virus infection. Our results reveal the sex-independent expression and tissue-specific processing of LsVg and also unprecedentedly connect the function of this protein in mediating virus transmission to its particular molecular forms existing in tissues previously known as non-Vg producing.
作者：Huo, Yan; Yu, Yuanling; Chen, Liying; 等
作者单位：中国科学院
期刊名称：PLOS PATHOGENS
期刊影响因子：6.158
出版年份：2018
出版刊次：2
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