中文摘要：越来越多的研究表明，包括南方水稻黑条矮缩病毒（SRBSDV）在内的植物病毒可通过未知机制对昆虫介体的生物学特性产生一些不利影响。为了研究SRBSDV在细胞水平上对昆虫介体的不利影响，作者通过破坏小干扰RNA（siRNA）途径促进病毒感染。利用透射电子显微镜来描述分析siRNA途径核心组分Dicer-2被敲除时昆虫体内发生的超微结构变化。SRBSDV在病毒感染的白背飞虱介体中的累积增加，导致消化道中出现了更严重的细胞病理变化。病毒侵染无效介体灰飞虱中肠超微结构也表现出了类似的细胞病理学变化。这些研究结果不仅支持了现有表明siRNA途径具有抗病毒作用的证据，而且还揭示了SRBSDV具有可对介体和非介体昆虫组织造成伤害的普遍和潜在能力。
外文摘要：An increasing number of studies are suggesting that plant viruses, including southern rice black-streaked dwarf virus (SRBSDV), can adversely affect biological characteristics of insect vectors by unknown mechanisms. To study the adverse effect of SRBSDV at cellular level on the insect vector, we promoted viral infection by the disruption of the small interfering RNA (siRNA) pathway. The transmission electron microscopy was utilized to describe the ultrastructural changes that occurred in insects when the core component of the siRNA pathway, Dicer-2, was knocked down. The increasing accumulation of SRBSDV in virus-infected vector, the white-backed planthoppers, caused severe cytopathology in the alimentary canal. Similar cytopathology changes in the midgut ultrastructure were characterized in the virus-infected incompetent vector, the small brown planthopper. These results not only add support to the existing evidence suggesting that the siRNA pathway has an antiviral effect, but also reveal the universal and potential ability of SRBSDV to cause damage to the insect tissues of both the vector and non-vector.
外文关键词：Southern rice black-streaked dwarf virus; Small interfering RNA; Pathogenicity; Planthopper
作者：Liu, Yuyan; Mao, Qianzhuo; Lan, Hanhong; 等
作者单位：福建农林大学
期刊名称：VIRUS RESEARCH
期刊影响因子：2.484
出版年份：2018
出版刊次：1
点击下载：飞虱Dicer-2基因敲除时消化道超微结构的调查研究揭示了南方水稻黑条矮缩病毒对其昆虫介体具有潜在致病性