中文摘要：了解基因功能的遗传和分子基础对于利用其潜在的能力生产具有新特征的植物具有越来越重要的意义。一个重要目标是提高植物生产力，以满足未来对于粮食作物的需求。基因功能主要通过转基因植物中的超表达或沉默来表征。这是一个漫长的过程，尤其是在谷物中。植物病毒表达系统可用于蛋白质的快速表达。然而，目前已有的系统载运量较小，且主要应用于基因沉默。本研究构建了具有高载运量的四元大麦条纹花叶病毒系统，用于不同植物中重组蛋白的快速和稳定表达，从而允许不同发育阶段的功能分析。
外文摘要：Understanding the genetic and molecular bases of gene function is of increasing importance to harness their potential to produce plants with novel traits. One important objective is the improvement of plant productivity to meet future demands in food crop production. Gene function is mostly characterized through overexpression or silencing in transgenic plants. This approach is a lengthy procedure, especially in cereals. Plant viral expression systems can be used for rapid expression of proteins. However, current systems have a small cargo capacity and have mostly been used for gene silencing. Here, a four-component barley stripe mosaic virus-based system with high cargo capacity was constructed for the rapid and stable expression of recombinant proteins in different plant species, allowing function analyses at different stages of development. Fluorescent marker proteins are expressed at high levels within 1 week, and a proof of efficient function analysis is shown using the aluminum malate transporter1 gene. In addition to the ability of gene cotransformation, this work demonstrates that the four-component barley stripe mosaic virus-based system allows the overexpression of cDNAs of up to 2,100 nucleotides (encoding a protein of similar to 78 kD), thereby providing an invaluable tool to accelerate functional genomics and proteomic research in monocot and dicot species.
作者：Cheuk, Arnaud; Houde, Mario
作者单位：University of Quebec Montreal
期刊名称：PLANT PHYSIOLOGY
期刊影响因子：5.949
出版年份：2018
出版刊次：3
点击下载：一种新的大麦条纹花叶病毒允许大蛋白质超表达进行快速功能分析的研究