中文摘要：水稻叶蝉是东南亚和中国南方一种主要的水稻害虫。必须探索新的控制策略来控制水稻害虫。通过调节肌钙蛋白C（TnC）来调节昆虫的性能或适应性可能是一种综合治理害虫的新策略。但是，TnC的特征与功能，尤其是关于其RNA干扰介导的基因敲除对水稻叶蝉性能或适应性的影响仍然未知。本研究成功地克隆和表征了水稻叶蝉的TnC基因。研究结果表明，Nc-TnC在成虫各个发育阶段和特有组织里不停地转录；Nc-TnC的瞬时敲除对水稻叶蝉的性能或适应性产生了负面影响，比如，存活率、取食能力、体重和繁殖力都会下降。研究还显示，Nc-TnC的表达受其与水稻矮缩病毒编码的非结构蛋白10的抑制，最终对水稻叶蝉的性能或适应性参数产生不利影响。总之，本研究数据加深了对Nc-TnC在水稻叶蝉生长发育及病毒感染过程中作用的理解。这意味着Nc-TnC将来可能会成为控制水稻叶蝉的一种潜在方法目标。
外文摘要：The green rice leafhopper, Nephotettix cincticeps, is a major rice pest in Southeast Asia and Southern China. Novel control strategies must be explored to control the rice pest. Behavior or fitness regulation of insect by modulating the Troponin C (TnC) may be a novel strategy in the comprehensive management of the insect pest. However, characterizations and functions of TnC, especially regarding effect of its RNA interference-mediated gene knockdown on the behavior or fitness of N. cincticeps remain unknown. Here, we successfully cloned and characterized TnC gene from N. cincticeps (Nc-TnC). We demonstrated that Nc-TnC ubiquitously transcribed at all development stages and special tissues in adult insects, with relative higher levels at the adult stage and in the intestinal canal. Microinjection or oral membrane feeding-based transient knockdown of Nc-TnC adversely affected the performance or fitness, such as the decreased survival, feeding capacity, weight, and fecundity of N. cincticeps. Furthermore, we revealed that the expression of Nc-TnC was suppressed by its interaction with rice dwarf virus-encoded nonstructural protein 10, which ultimately affected detrimentally the corresponding parameters of the performance or fitness of N. cincticeps. In conclusion, our data deepen understanding of Nc-TnC functions during the development of and viral infection in N. cincticeps. It imply Nc-TnC may serve as a potential target for N. cincticeps control in future.
外文关键词：expression profiles; interaction; Nephotettix cincticeps; performance or fitness; rice dwarf virus; RNAi; Troponin C
作者：Lan, Hanhong; Hong, Xiaojing; Huang, Ranran; 等
作者单位：闽南师范大学
期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
期刊影响因子：1.316
出版年份：2018
出版刊次：2
点击下载：RNA干扰介导肌钙蛋白C基因的敲除和病毒诱导肌钙蛋白C基因的抑制对水稻叶蝉的性能或适应性产生不利影响