中文摘要：番茄黄化曲叶病毒（TYLCV）是最重要的植物病害之一，由烟粉虱传播。TYLCV是番茄上一种毁灭性的病害，严重影响了受感染番茄植株的生长。MYB转录因子在植物发育和病原体防御反应中起着重要作用，但他们在番茄抵抗TYLCV侵染反应中的作用仍未完全了解。本研究鉴定了一个番茄MYB基因，即SlMYB28。研究发现，在TYLCV敏感品种'金鹏-1'和TYLCV抗性品种'Zheza-301'中，TYLCV感染强烈诱导了SlMYB28的表达。通过使用病毒诱导的基因沉默进一步研究了SIMYB28对TYLCV的反应的功能，在SlMYB28沉默的番茄植株中TYLCV的DNA含量低于对照。这些结果意味着SlMYB28在番茄TYLCV感染调节中起着一个负向调节因子的作用。
外文摘要：Tomato yellow leaf curl virus (TYLCV), which is one of the most critical plant diseases, is transmitted by Bemisia tabaci. TYLCV is a devastating disease of tomato, senously affecting the growth of infected tomato plants. MYB transcription factors play central roles in plant development and pathogen defense response, but their functions in tomato defense response to TYLCV infection remain incompletely understood. In this study, a tomato MYB gene, SlMYB28 was identified. The open reading frame of SlMYB28 gene spans 702 bp, encoding 233 amino acids. SlMYB28 is an R2R3-MYB transcription factor Nuclear localization assays revealed that SlMYB28 protein localizes to the nucleus. In the promoter region of SlMYB28 gene, a few cis-elements related to plant development or environmental stress responses were observed. SlMYB28 expression was strongly induced by TYLCV infection in TYLCV-susceptible cultivar 'Jinpeng-l'and TYLCV-resistant cultivar 'Zheza-301'. We further investigated the function of SIMYB28 in response to TYLCV by using virus-induced gene silencing. DNA content of TYLCV in SlMYB28-silenced tomato plant was lower than that of the negative control line. These results imply the involvement of SlMYB28 as a negative regulator in regulation of TYLCV infection in tomato.
外文关键词：MYB; Transcription factor; TYLCV; VIGS; Defense stress response; Tomato
作者：Li, Tong; Zhang, Xin-Yue; Huang, Ying; 等
作者单位：南京农业大学
期刊名称：SCIENTIA HORTICULTURAE
期刊影响因子：1.76
出版年份：2018
出版刊次：7
点击下载：R2R3-MYB转录因子S1MYB28参与番茄中TYLCV侵染调节的研究