中文摘要：番茄萎黄病毒（ToCV）是巴西新出现的一种毛形病毒属病毒，可在番茄和其他茄科作物上引起严重的经济性病害。ToCV主要由烟粉虱(MEAM1, 以前为B型)以半持久性方式传播。由于所有番茄栽培种和杂交种都对这种毛形病毒属病毒敏感，控制该病害的主要可选择办法是使用健康的幼苗进行移植以及对昆虫介体进行化学控制。本研究目的是评估番茄基因型对毛形病毒属病毒侵染的反应及其对该病害的耐受性，从而为控制病害的其他可选择办法的开发提供支持。通过带毒MEAM1烟粉虱接种ToCV，随后采用RT-PCR和RT-qPCR检测病毒，从而评估番茄基因型对病毒侵染的抗性。为了测量对病害的耐受性，对感染ToCV植株和健康植株的植物发育和果实产量进行了比较。56个基因型中只有IAC-CN-RT品系对ToCV感染具有高度抗性。不同基因型的两个试验中对病害的耐受性显示出不同的结果。高抗ToCV的IAC-CN-RT品系对西班牙ToCV分离株也有抗性，这可能有助于番茄育种计划。
外文摘要：Tomato chlorosis virus (ToCV) is an emerging crinivirus in Brazil that causes an economically important disease in tomato (Solanum lycopersicum) and other solanaceous species. ToCV is transmitted predominantly by the whitefly Bemisia tabaci Middle East-Asia Minor 1 (MEAM1, formerly biotype B), in a semipersistent manner. As all cultivated tomato varieties and hybrids are susceptible to this crinivirus, the main alternatives for the control of the disease are the use of healthy seedlings for transplanting and the chemical control of the insect vector. The objective of this work was to evaluate the responses of tomato genotypes to infection with this crinivirus and their tolerance to the disease in order to support the development of other alternatives for disease control. Resistance to infection was evaluated by ToCV inoculation with viruliferous B. tabaciMEAM1 followed by virus detection by RT-PCR and RT-qPCR. To measure tolerance to the disease, plant development and fruit yield of ToCV-infected and healthy plants were compared. Among 56 genotypes, only the lineage IAC-CN-RT (S. lycopersicum Angela Gigante'xS. peruvianum LA 444-1') was highly resistant to infection with ToCV. Tolerance to the disease over two trials with different genotypes showed variable results. The effect of ToCV on plant development varied from 2.9% to 71.9% reduction, while yield loss varied from 0.2% to 51.8%. The highly ToCV-resistant lineage IAC-CN-RT, which is also resistant to a Spanish isolate of ToCV, might be useful for tomato breeding programmes.
外文关键词：Bemisia tabaci; crinivirus; Solanum peruvianum
作者：Mansilla-Cordova, P. J.; Bampi, D.; Rondinel-Mendoza, N. V.; 等
作者单位：巴西圣保罗大学
期刊名称：PLANT PATHOLOGY
期刊影响因子：2.303
出版年份：2018
出版刊次：6
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