中文摘要：分泌到细胞壁和细胞外空隙的蛋白质在植物发育、代谢和胁迫反应中具有不同的作用。先前已有研究对辣椒-病原体相互作用过程中分泌的蛋白质进行分离，通过实证阐明其作用。在这些蛋白质中，作者进一步表征了脂氧合酶同源基因CaLOP，该同源基因含有730bp的cDNA序列并编码184个氨基酸。CaLOP含有脂氧合酶同源性（PLAT/LH2）结构域。为了检验该基因在植物发育中的生物学功能，本研究在植物敲除实验中采用了基于烟草脆裂病毒的基因沉默系统。与对照相比，辣椒中CaLOP沉默导致了异常生长和严重的发育障碍，包括致死表型。研究结果表明，CaLOP可能在植物生长和发育以及病原体防御中起着重要作用。
外文摘要：The proteins secreted into the cell wall and extracellular space have multiple roles in plant development, metabolism, and stress responses. In a previous study, examples of such proteins secreted during pepper-pathogen interactions were isolated to elucidate their roles. Among these proteins, we further characterized a lipoxygenase homology gene CaLOP (Capsicum annuum lipoxygenase homology protein), which has a 730 bp cDNA sequence and encodes 184 amino acids. CaLOP contains a lipoxygenase homology (PLAT/LH2) domain. A database search revealed that CaLOP shares significant sequence identity with putative orthologs in other plants. CaLOP was constitutively expressed in root tissue, and was upregulated in response to TMV-P0, TMV-P2, and PepMoV viruses, as well as Phytophthora capsici. To examine the biological function of this gene in plant development, we performed in planta knockdown assays using the Tobacco rattle virus-based gene-silencing system. Silencing CaLOP in pepper resulted in abnormal growth and severe developmental disorders, including lethal phenotypes, compared with controls. Consequently, our results suggest that CaLOP may be important in plant growth and development, and pathogen defense.
外文关键词：Capsicum; lipoxygenase-homology protein; secreted protein; VIGS
作者：Kang, Won-Hee; Lee, Junesung; Nam, Jae-Young; 等
作者单位：韩国庆尚大学
期刊名称：HORTICULTURAL SCIENCE & TECHNOLOGY
期刊影响因子：0.0
出版年份：2018
出版刊次：2
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