中文摘要：水稻条纹病毒（RSV）属于纤细病毒属，由灰飞虱以持久、循环增殖型方式传播，可引起水稻条纹病（RSD）。由RNA3病毒链编码的RSV的NS3蛋白是RNA沉默病毒抑制子（VSR）。NS3在病毒感染中起着重要的作用，且NS3转基因植物表现出对病毒的抗性。本研究利用northern blot分析法对转基因烟草产生的NS3的稳定性和有效性进行研究。通过western blot分析法对病毒的积累进行检测。利用转录组测序鉴定NS3-转基因烟草中的差异表达基因（DEGs）。研究结果表明，NS3在病毒侵染过程中起双重作用，是RSV感染过程中的一个RNA沉默病毒抑制子（VSR），并在转基因寄主植物中实现了病毒抗性。该研究增强了我们对VSR与寄主植物之间交互作用的理解。
外文摘要：Background: Rice stripe virus (RSV) belongs to the genus Tenuivirus. It is transmitted by small brown planthoppers in a persistent and circulative-propagative manner and causes rice stripe disease (RSD). The NS3 protein of RSV, encoded by the viral strand of RNA3, is a viral suppressor of RNA silencing (VSR). NS3 plays a significant role in viral infection, and NS3-transgenic plants manifest resistance to the virus. Methods: The stability and availability of NS3 produced by transgenic Nicotiana benthamiana was investigated by northern blot analysis. The accumulation of virus was detected by western blot analysis. Transcriptome sequencing was used to identify differentially expressed genes (DEGs) in NS3-transgenic N. benthamiana. Results: When the host plants were inoculated with RSV, symptoms and viral accumulation in NS3-transgenic N. benthamiana were reduced compared with the wild type. Transcriptome analysis identified 2533 differentially expressed genes (DEGs) in the NS3-transgenic N. benthamiana, including 597 upregulated genes and 1936 downregulated genes. These DEGs were classified into three Gene Ontology (GO) categories and were associated with 43 GO terms. KEGG pathway analysis revealed that these DEGs were involved in pathways associated with ribosomes (ko03010), photosynthesis (ko00195), photosynthesis-antenna proteins (ko00196), and carbon metabolism (ko01200). More than 70 DEGs were in these four pathways. Twelve DEGs were selected for RT-qPCR verification and subsequent analysis. The results showed that NS3 induced host resistance by affecting host gene expression. Conclusion: NS3, which plays dual roles in the process of infection, may act as a VSR during RSV infection, and enable viral resistance in transgenic host plants. NS3 from RSV affects the expression of genes associated with ribosomes, photosynthesis, and carbon metabolism in N. benthamiana. This study enhances our understanding of the interactions between VSRs and host plants.
外文关键词：Rice stripe virus; NS3; Suppressor of RNA silencing; Nicotiana benthamiana; Transcriptomes
作者：Wu, Gentu; Zheng, Guixian; Hu, Qiao; 等
作者单位：浙江农业科学院
期刊名称：VIROLOGY JOURNAL
期刊影响因子：2.465
出版年份：2018
出版刊次：6
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