中文摘要：在植物中，RNA沉默是抗病毒免疫潜在的保守机制。为了研究烟草中的抗病毒反应，本文分析了野生型烟草中病毒衍生小RNAs (vsRNAs)和被黄瓜花叶病毒（CMV）2b缺失突变体感染的NbRDR6突变植株图谱。研究观察到NbRDR6通过产生vsRNAs触发有效的抗病毒反应来调节RNA沉默，但是NbRDR1可非冗余地与NbRDR6协同作用以介导免疫反应。研究结果表明，NbAGO2与vsRNAs的抗病毒活性密切相关，烟草具有特异的和保守的抗病毒免疫力。
外文摘要：In plants, RNA silencing is a conserved mechanism underlying antiviral immunity. To investigate antiviral responses in Nicotiana benthamiana, we analyzed the profiles of the virus-derived small RNAs (vsRNAs) in wild type N. benthamiana and NbRDR6 mutant plants infected with the cucumber mosaic virus (CMV) 2b-deficient mutant. We observed that NbRDR6 regulates RNA silencing by producing vsRNAs that trigger an effective antiviral response, while NbRDR1 may nonredundantly and synergistically function with NbRDR6 to mediate immune responses. The vsRNAs in N. benthamiana and NbRDR6 mutant plants mainly comprised 21 or 22 nucleotides, and mostly consisted of a 5'-terminal adenine. Additionally, NbAGO2 expression was significantly up regulated in N. benthamiana and NbRDR6 mutant plants, suggesting that NbAGO2 is closely associated with the antiviral activities of vsRNAs. The distribution of vsRNAs in the CMV genome was biased toward RNA sense strands in both N. benthamiana and NbRDR6 mutant plants. These findings indicate the specific and conserved antiviral immunity in Nicotiana benthamiana.
外文关键词：RNA silencing; Deep sequencing; Virus-derived small RNAs; Cucumber mosaic virus
作者：Qiu, Yanhong; Wu, Yuping; Zhang, Yongjiang; 等
作者单位：中国检验检疫科学研究院
期刊名称：VIRUS RESEARCH
期刊影响因子：2.484
出版年份：2018
出版刊次：7
点击下载：通过深度测序分析源自于受感染烟草植株中黄瓜花叶病毒的小RNAs图谱