中文摘要：番茄黄化曲叶病毒（TYLCV）已对世界各地番茄市场产生了重大影响，使用杀虫剂和防虫网已不能有效控制该病原体的传播。番茄品系AVTO1227是一种高抗TYLCV的材料。本文利用AVTO1227和感病株系“Money maker”构建了F2和BC1群体，用来评估TYLCV抗性遗传机制。研究鉴定到一个和SlNACI连锁的隐性TYLCV抗性基因ty-5。构建来自抗性和易感番茄组的基因组DNA库，并对其基因组重新测序。研究选定将pelota基因作为ty-5的候选基因。但研究结果表明，无论是否感染TYLCV，三个番茄品系中的pelota相关转录水平都没有显着差异。本究将有助于标记辅助TYLCV抗性育种，为研究番茄中ty-5的抗性机制奠定基础。
外文摘要：Tomato yellow leaf curl virus (TYLCV) has significantly impacted the tomato industry around the world, and the use of insecticides and insect nets have not effectively controlled the spread of this pathogen. The tomato line AVTO1227 is highly resistant to TYLCV. In this study, F-2 and BC1 populations derived from AVTO1227 and the susceptible line Money maker were used to assess the genetic mechanism underlying TYLCV resistance. We have identified a recessive TYLCV resistance gene, hereby designated as ty-5, which is linked to SINACI. Genomic DNA pools from resistant and susceptible groups were constructed, and their genomes were resequenced. The ty-5 gene was identified on an interval encompassing the genomic positions 2.22 Mb to 3.19 Mb on tomato chromosome 4. Genotyping using linkage markers further mapped ty-5 within the interval between markers ty5-25 and ty5-29, where only the pelota gene is located. Consequently, pelota was considered as the candidate gene corresponding to ty-5. Two nucleotide transversions within the promoter region and one transversion in exon region of the pelota gene were detected in the parental lines. However, the relative transcript levels of pelota did not significantly differ among the three tomato lines, regardless of TYLCV infection. This study will facilitate marker-assisted breeding for resistance to TYLCV and lay a foundation for the research of the resistance mechanism of ty-5 in tomato.
作者：Wang, Yinlei; Jiang, Jing; Zhao, Liping; 等
作者单位：江苏省农业科学院
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.122
出版年份：2018
出版刊次：6
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