中文摘要：番茄黄化曲叶病毒可引起番茄黄化曲叶病，导致全球范围内番茄产量显著降低。已提倡使用抗性品种作为可持续管理策略。在遗传上表征番茄抗性基因（R基因）方面已经取得了巨大进展。目前已有的TYLCV抗性番茄育种主要是基于将来自于野生番茄的Ty-3作为特异性抗性基因进行基因渗入。通过使用标记辅助选择法（MAS）可以实现品种的改良或发育。因此，精确简单地使用基因靶向标记对于育种计划的选择非常重要。本研究旨在开发一种基于Ty-3基因序列的新标记，该标记可用于TYLCV抗性番茄育种计划中的MAS。新开发的标记被命名为ACY。通过筛选商业抗性和易感番茄杂交品种，以及使用来自商业抗性杂种AG208的F2代遗传分离，评估ACY及Ty-3连锁标记P6-25的可靠性和准确性。研究获得的结果与单个抗性显性基因遗传的3:1分离比相一致，这说明了ACY为功能基因标记。目前该标记已在江苏绿港现代农业开发公司用于筛选968个杂交品种以及1000个番茄品种的品系育种。到目前为止，ACY已用于鉴定56个杂交种和51个育种品系。这些新发现的育种品系被认为是番茄育种潜在的抗性来源。本研究开发了Ty-3的序列，促进了分子标记ACY的发展，从而有助于表型的选择。因此，可向育种者推荐这种适用于番茄标记辅助选择的标记。
外文摘要：Tomato yellow leaf curl virus (TYLCV) responsible for tomato yellow leaf curl disease (TYLCD) causes a substantial decrease in tomato (Solanum lycopersicum L.) yield worldwide. The use of resistant variety as a sustainable management strategy has been advocated. Tremendous progress has been made in genetically characterizing the resistance genes (R gene) in tomato. Breeding tomato for TYLCV resistance has been based mostly on Ty-3 as a race-specific resistance gene by introgression originating from wild tomato species relatives. Improvement or development of a cultivar is achievable through the use of marker-assisted selection (MAS). Therefore, precise and easy use of gene-targeted markers would be of significant importance for selection in breeding programs. The present study was undertaken to develop a new marker based on Ty-3 gene sequence that can be used for MAS in TYLCV resistant tomato breeding program. The new developed marker was named ACY. The reliability and accuracy of ACY were evaluated against those of Ty-3 linked marker P6-25 through screening of commercial resistant and susceptible tomato hybrids, and genetic segregation using F2 population derived from a commercial resistant hybrid AG208. With the use of bioinformatics and DNA sequencing analysis tools, deletion of 10 nucleotides was observed in Ty-3 gene sequence for susceptible tomato variety. ACY is a co-dominant indel-based marker that produced clear and strong polymorphic band patterns for resistant plant distinguishing it from its susceptible counterpart. The obtained result correlates with 3:1 segregation ratio of single resistant dominant gene inheritance, which depicted ACY as gene-tag functional marker. This marker is currently in use for screening 968 hybrids varieties and one thousand breeding lines of tomato varieties stocked in Jiangsu Green Port Modern Agriculture Development Company (Green Port). So far, ACY has been used to identify 56 hybrids and 51 breeding lines. These newly detected breeding lines were regarded as potential source of resistance for tomato breeding. This work exploited the sequence of Ty-3 and subsequently contributed to the development of molecular marker ACY to aid phenotypic selection. We thus recommend this marker to breeders, which is suitable for marker-assisted selection in tomato.
作者：Nevame, Adedze Yawo Mawunyo; Xia, Lu; Nchongboh, Chofong Gilbert; 等
作者单位：Jiangsu Green Port Modern Agr Dev Co
期刊名称：BIOMED RESEARCH INTERNATIONAL
期刊影响因子：2.583
出版年份：2018
出版刊次：8
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