中文摘要：菜豆金黄花叶病毒属（双生病毒）经常与旧世界的α卫星和 β卫星相关联。番茄黄化曲叶病毒是世界上最具破坏性的病毒种类之一，最近发现它与地中海东海岸和中东地区的β卫星有关。在实验条件下，番茄黄化曲叶病毒（TYLCV）/β卫星关联显示出增加TYLCV毒力。本文通过评估比较卫星和TYLCV的积累水平、介体传播效率，以及通过检测地中海盆地大多数TYLCV抗性番茄品种中使用的Ty-1抗性基因多远对β卫星有效，来评估番茄中TYLCV/卫星关联的持续性。本研究结果表明，β卫星对番茄栽培（包括具有Ty-1抗性的栽培种）具有潜在的风险。
外文摘要：Begomoviruses (family Geminiviridae) are frequently associated with alphasatellites and betasatellites in the Old World. Tomato yellow leaf curl virus, one of the most damaging begomovirus species worldwide, was recently found associated with betasatellites in the eastern coast of the Mediterranean Sea, and in the Middle East region. Tomato yellow leaf curl virus (TYLCV)/betasatellite associations were shown to increase TYLCV virulence in experimental conditions. The sustainability of TYLCV/satellite associations in tomato was assessed here by estimating accumulation levels of satellites in comparison to TYLCV, vector transmission efficiency, and by testing how far the popular Ty-1 resistance gene used in most TYLCV-resistant tomato cultivars in the Mediterranean Basin is effective against betasatellites. Three satellites previously isolated from okra in Burkina Faso of the species Cotton leaf curl Gezira betasatellite, Cotton leaf curl Gezira alphasatellite and Okra leaf curl Burkina Faso alphasatellite were shown to accumulate at levels similar to, or higher than, the helper virus TYLCV-Mld in tomato plants from 32 to 150 days post inoculation (dpi). Cotton leaf curl Gezira betasatellite (CLCuGB) reduced TYLCV-Mld accumulation whereas alphasatellites did not. Transmission tests were performed with B. tabaci from plants infected with TYLCV-Mld/CLCuGB- or TYLCV-Mld/Okra leaf curl Burkina Faso alphasatellite. At 32 dpi, both satellites were transmitted to more than 50% of TYLCV-infected test plants. Betasatellite transmission, tested further with 150 dpi source plants was successful in more than 30% of TYLCV-infected test plants. Ty-1 resistant tomato plants co-infected with TYLCV (-Mld or -IL) and CLCuGB exhibited mild leaf curling and mosaic symptoms at the early stage of infection associated with a positive effect on TYLCV-IL accumulation, while resistant plants infected with TYLCV only, were asymptomatic. Together with previous experimental studies, these results further emphasize the potential risk of betasatellites to tomato cultivation, including with Ty-1 resistant cultivars.
外文关键词：Begomovirus; Geminiviridae; DNA satellites; Real time PCR; Bemisia tabaci
作者：Conflon, Deborah; Granier, Martine; Tiendrebeogo, Fidele; 等
作者单位：CIRAD
期刊名称：VIRUS RESEARCH
期刊影响因子：2.484
出版年份：2018
出版刊次：7
点击下载：α卫星、 β卫星和番茄黄化曲叶病毒在易感和Ty-1抗性番茄植株中的积累和传播