中文摘要：印度尼西亚是世界上最大的新鲜水果辣椒生产国之一，辣椒是印度尼西亚美食中必不可少的调料。在过去的二十年中，菜豆金黄花叶病毒属病毒由烟粉虱传播，可引起辣椒黄花曲叶病，通过破坏印度尼西亚的辣椒植株造成巨大的经济损失。本研究通过农杆菌接种和随后的嫁接法相结合，建立了一种印度尼西亚辣椒黄化曲叶病毒（PepYLCIV，北苏门答腊种植的辣椒中最具侵染性的双生病毒）高效接种法。
外文摘要：Indonesia is one of the world's largest fresh pepper (Capsicum spp.) fruit-producing countries, and hot peppers are essential spices in Indonesian cuisine. During the last two decades, begomovirus, which is transmitted by the whitefly, Bemisia tabaci (Gennadius), and causes pepper yellow leaf curl disease, began to cause a huge economic loss by damaging pepper plants in Indonesia. In the present study, a highly efficient inoculation method was established for Pepper yellow leaf curl Indonesia virus (PepYLCIV), the most infectious bipartite begomovirus in pepper plants cultivated in North Sumatra, by combining agroinoculation and subsequent grafting. Partial tandem repeats of PepYLCIV DNA A and B were constructed and cloned into a binary pGreenII vector, and their infectivity was tested. Co-inoculation of Nicotiana benthamiana L. and Solanum lycopersicum L. 'Momotaro' with PepYLCIV DNA A and DNA B resulted in the production of typical begomoviral symptoms. Both the injection of the cotyledons with cultured agrobacteria and the inoculation of the hypocotyl with agrobacterial colonies induced viral symptoms in pepper No. 218 (C. annuum L.) seedlings in approximately 55-75%. When agroinoculated symptomatic No. 218 was grafted onto an uninfected 'Takanotsume' (C. annuum), all newly elongated shoots from the rootstock of 'Takanotsume' produced typical begomoviral symptoms. Agroinoculation combined with subsequent grafting provides a highly efficient method for introducing PepYLCIV into pepper plants.
外文关键词：begomovirus; geminivirus; infectious clone; PepYLCIV; Southeast and East Asia
作者：Koeda, Sota; Homma, Kanami; Tanaka, Yuri; 等
作者单位：近畿大学
期刊名称：HORTICULTURE JOURNAL
期刊影响因子：1.035
出版年份：2018
出版刊次：7
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