中文摘要：水稻条纹病毒（RSV）属于纤细病毒属。该病毒由灰飞虱以持久和循环增殖型的方式传播并引起水稻条纹病（RSD）。RSV 的NS3蛋白，由RNA3病毒链编码，是RNA沉默的病毒抑制因子（VSR）。NS3在病毒侵染中起着重要作用，NS3-转基因植物表现出病毒抗性。本研究结果表明：NS3可通过影响寄主基因表达诱导寄主抗性。可见，NS3在感染过程中起双重作用，在RSV侵染期间可作为VSR，并在转基因寄主植物中实现病毒抗性。本研究有助于增强对VSRs与寄主植物之间交互作用的理解。
外文摘要：Background: Rice stripe virus (RSV) belongs to the genus Tenuivirus. It is transmitted by small brown planthoppers in a persistent and circulative-propagative manner and causes rice stripe disease (RSD). The NS3 protein of RSV, encoded by the viral strand of RNA3, is a viral suppressor of RNA silencing (VSR). NS3 plays a significant role in viral infection, and NS3-transgenic plants manifest resistance to the virus. Methods: The stability and availability of NS3 produced by transgenic Nicotiana benthamiana was investigated by northern blot analysis. The accumulation of virus was detected by western blot analysis. Transcriptome sequencing was used to identify differentially expressed genes (DEGs) in NS3-transgenic N. benthamiana. Results: When the host plants were inoculated with RSV, symptoms and viral accumulation in NS3-transgenic N. benthamiana were reduced compared with the wild type. Transcriptome analysis identified 2533 differentially expressed genes (DEGs) in the NS3-transgenic N. benthamiana, including 597 upregulated genes and 1936 downregulated genes. These DEGs were classified into three Gene Ontology (GO) categories and were associated with 43 GO terms. KEGG pathway analysis revealed that these DEGs were involved in pathways associated with ribosomes (ko03010), photosynthesis (ko00195), photosynthesis-antenna proteins (ko00196), and carbon metabolism (ko01200). More than 70 DEGs were in these four pathways. Twelve DEGs were selected for RT-qPCR verification and subsequent analysis. The results showed that NS3 induced host resistance by affecting host gene expression. Conclusion: NS3, which plays dual roles in the process of infection, may act as a VSR during RSV infection, and enable viral resistance in transgenic host plants. NS3 from RSV affects the expression of genes associated with ribosomes, photosynthesis, and carbon metabolism in N. benthamiana. This study enhances our understanding of the interactions between VSRs and host plants.
外文关键词：Rice stripe virus, NS3, Suppressor of RNA silencing, Nicotiana benthamiana, Transcriptomes
作者：Wu, Gentu; Zheng, Guixian; Hu, Qiao; 等
作者单位：西南大学
期刊名称：VIROLOGY JOURNAL
期刊影响因子：2.465
出版年份：2018
出版刊次：6
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