中文摘要：南方水稻黑条矮缩病毒（SRBSDV）是呼肠孤病毒科斐济病毒属，由于缺乏有效的控制措施，可能会导致严重的作用损失。嘧肽霉素是一种由中国开发的微生物农药，对许多植物病毒具有广泛的抗病毒活性，但其潜在机制仍不清楚。本研究使用无标记蛋白质组学技术鉴定了共2321种蛋白质。与 SRBSDV感染的水稻处理相比，在预先用嘧肽霉素处理过的感染SRBSDV的水稻处理中检测到84和207个蛋白质上调。同时，实时定量PCR（RT-qPCR）嘧肽霉素也可上调一些抗性基因，这些结果表明与无标记蛋白质组的数据趋势相似。此外，嘧肽霉素可增强POD、SOD、CAT的抗性酶活性。这些数据提供了一个更全面观点：嘧肽霉素可触发感染了SRBSDV的水稻在蛋白质组、mRNA和酶活性水平上的响应。
 
外文摘要： developed by China, displayed a wide antiviral activity against many plant viruses. However, its underlying mechanism remains unclear. In this study, a total of 2321 proteins were identified using label-free proteomics technology. Compared with the treatment of SRBSDV-infected rice, 84 and 207 proteins were detected to be up-regulated and only presented in treatment group of SDBSDV-infected rice pre-treated by Cytosinpeptidemycin, which were partially enriched to stress and defense response, such as pathogenesis-related protein 5 (PR-5), pathogenesis-related protein 10 (PR-10) and heat shock protein (Hsp protein). Meanwhile, the real-time quantitative PCR (RT-qPCR) showed that Cytosinpeptidemycin could also up-regulate some resistance genes, and these results indicated a similar trends with the data of the label-free proteomics. Moreover, Cytosinpeptidemycin could enhance the defense enzymatic activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). These data offer a more comprehensive view about the response of SRBSDV-infected rice triggered by Cytosinpeptidemycin in the level of the proteome, mRNA and enzymatic activity. (C) 2017 Elsevier Inc. All rights reserved.
外文关键词：Cytosinpeptidemycin; SRBSDV; Plant resistance; Label-free proteomic; RT-qPCR; Enzymatic activity
作者：Yu, Lu; Wang, Wenli; Zeng, Song; 等
作者单位：贵州大学
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
期刊影响因子：3.44
出版年份：2018
出版刊次：5
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