中文摘要：2014年，埃及感染番茄的番茄黄化曲叶病毒（TYLCV）广泛传播。 对来自不同省份的感染了TYLCV病毒的番茄植株进行了取样。通过多重实时PCR法鉴定TYLCV以色列菌株和TYLCV米尔德菌株（TYLCV-IL，TYLCV-Mild）。此外，VI和V2蛋白基因的核苷酸序列分析揭示了10个TYLCV埃及分离菌株（TY1 to TY10）。系统发育分析显示，它们与TYLCV-IL约旦分离株具有高度关联性（98%）。本文揭示了从埃及布海拉省取样所得的TYLCV埃及分离株TY10的完整核苷酸序列。与先前报道的埃及分离株以及约旦和日本等其他分离株的高度相似性体现了系统发育分析在监测病毒遗传多样性以及更多病毒菌株或基因型分化可能性中的重要性。
外文摘要：Tomato yellow leaf curl virus (TYLCV) infections of tomato crops in Egypt were widely spread in 2014. Infected symptomatic tomato plants from different governorates were sampled. TYLCV strains Israel and Mild (TYLCV-IL, TYLCV-Mild) were identified by multiplex and real-time PCR. In addition, nucleotide sequence analysis of the VI and V2 protein genes, revealed ten TYLCV Egyptian isolates (TYLCV from TY1 to 10). Phylogenetic analysis showed their high degree of relatedness with TYLCV-IL Jordan isolate (98%). Here we have showed the complete nucleotide sequence of the TYLCV Egyptian isolate TY10, sampled from El Beheira. A high degree of similarity to other previously reported Egyptian isolates and isolates from Jordan and Japan reflect the importance of phylogenetic analysis in monitoring virus genetic diversity and possibilities for divergence of more virulent strains or genotypes.
外文关键词：multiplex PCR; real-time PCR; TYLCV-IL; TYLCV-Mild; phylogenetic analysis
作者：Rabie, M.; Ratti, C.; Aleem, E. Abdel; 等
作者单位：亚历山大大学
期刊名称：ACTA VIROLOGICA
期刊影响因子：0.696
出版年份：2017
出版刊次：9