中文摘要：玉米粗缩病（MRDD）是一种在中国南方和其他许多亚洲国家频繁发生的严重疾病。MRDD是由水稻黑条矮缩病毒（RBSDV）的感染引起的，并会导致玉米生产发生显著的经济损失。为了更好地理解RBSDV感染对玉米生长的破坏作用，使用LC-MS/MS和TMT标记相整合的方法对感染RBSDV的玉米幼苗进行比较蛋白组学分析。研究发现：共有7615个玉米蛋白，其中6319个被量化。鉴定了116个差异富集蛋白（DAPs），包括RBSDV感染后35种上调蛋白和81种下调蛋白。富集分析显示，DAPs与氰氨基酸代谢，ER蛋白加工和核糖体相关途径最为强烈相关。研究结果还表明，硫可能参与了抗RBSDV感染。本研究数据显示，玉米对RBSDV侵染的反应由多种不同的代谢途径控制。
外文摘要：Background: Maize rough dwarf disease (MRDD) is a severe disease that has been occurring frequently in southern China and many other Asian countries. MRDD is caused by the infection of Rice black streaked dwarf virus (RBSDV) and leads to significant economic losses in maize production. To well understand the destructive effects of RBSDV infection on maize growth, comparative proteomic analyses of maize seedlings under RBSDV infection was performed using an integrated approach involving LC-MS/MS and Tandem Mass Tag (TMT) labeling. Results: In total, 7615 maize proteins, 6319 of which were quantified. A total of 116 differentially accumulated proteins (DAPs) were identified, including 35 up-and 81 down-regulated proteins under the RBSDV infection. Enrichment analysis showed that the DAPs were most strongly associated with cyanoamino acid metabolism, protein processing in ER, and ribosome-related pathways. Two sulfur metabolism-related proteins were significantly reduced, indicating that sulfur may participate in the resistance against RBSDV infection. Furthermore, 15 DAPs involved in six metabolic pathways were identified in maize under the RBSDV infection. Conclusions: Our data revealed that the responses of maize to RBSDV infection were controlled by various metabolic pathways.
外文关键词：Maize rough dwarf disease; Rice black streaked dwarf virus; Differentially accumulated proteins; Metabolism; Maize
作者：Yue, Runqing; Lu, Caixia; Han, Xiaohua; 等
作者单位：河南省农业科学研究院
期刊名称：BMC PLANT BIOLOGY
期刊影响因子：3.93
出版年份：2018
出版刊次：9
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