中文摘要：番茄黄化曲叶病毒（TYLCV）是一种病毒病，可在全球番茄上流行。之前已有的许多研究主要集中在筛选野生番茄品种作为抗性资源。在许多情况下，筛选出的番茄品种既没有公开也没有连贯性，这导致了许多多余、重复的筛选工作。本研究中作者努力从各种已公开的资源中筛选野生番茄TYLCV抗性品种，通过应用病毒诱导的基因沉默和等位基因挖掘技术研究了S. chilense 中Ty1/Ty-3基因的等位变异，从而鉴定许多等位基因特异性多态性。总而言之，本研究提供了一个研究野生番茄种质对TYLCV抗性和易感性的全面的视角，并展示了如何研究TYLCV抗性种质中克隆的Ty基因的等位基因变体。
外文摘要：Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publications or not provided in a consistent manner, which led to redundant screenings. In the current study, we summarized efforts on the screenings of wild tomato species for TYLCV resistance from various publications. In addition, we screened 708 accessions from 13 wild tomato species using different inoculation assays (i.e., whitefly natural infection and Agrobacterium-mediated inoculation) from which 138 accessions exhibited no tomato yellow leaf curl disease (TYLCD) symptoms. These symptomless accessions include 14 accessions from S. arcanum, 43 from S. chilense, 1 from S. chmielewskii, 28 from S. corneliomulleri, 5 from S. habrochaites, 4 from S. huaylasense, 2 from S. neorickii, 1 from S. pennellii, 39 from S. peruvianum, and 1 from S. pimpinellifolium. Most of the screened S. chilense accessions remained symptomless. Many symptomless accessions were also identified in S. arcanum, S. corneliomulleri, and S. peruvianum. A large number of S. pimpinellifolium accessions were screened. However, almost all of the tested accessions showed TYLCD symptoms. Further, we studied allelic variation of the Ty1/Ty-3 gene in few S. chilense accessions by applying virus-induced gene silencing and allele mining, leading to identification of a number of allele-specific polymorphisms. Taken together, we present a comprehensive overview on TYLCV resistance and susceptibility in wild tomato germplasm, and demonstrate how to study allelic variants of the cloned Ty-genes in TYLCV-resistant accessions.
外文关键词：Begomovirus; resistance; Solanum lycopersicum; S. chilense; S. peruvianum; tomato; TYLCV
作者：Yan, Zhe; Perez-de-Castro, Ana; Diez, Maria J.; 等
作者单位：中国农业科学院
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：3.678
出版年份：2018
出版刊次：8
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