中文摘要：玉米粗缩病玉米中由水稻黑条矮缩病毒（RBSDV）引起的一种破坏性病害。已知小RNA（miRNAs）在调节植物生长、发育及适应非生物和生物胁迫时起着关键作用。为了阐明miRNAs在调节玉米对RBSDV响应中的作用，本研究采用高通量测序技术分析了RBSDV 侵染后的miRNAome和转录组。共鉴定了76种已知的miRNAs，226种潜在的新miRNAs和351个靶基因。数据显示，在响应RBSDV侵染时81个miRNAs的表达模式发生了剧烈变化。转录组分析显示，RBSDV侵染后453个基因差异表达。GO、COG 和KEGG分析结果表明，与光合作用和新陈代谢相关的基因显著富集。此外，鉴定了12对miRNA-mRNA交互作用搭档，其中有6对可能在玉米响应RBSDV中具有重要作用。该研究为理解玉米抗病性的分子机制提供了有价值的信息，可用于开发保护玉米免受RBSDV侵害的方法。
外文摘要：Maize rough dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is a devastating disease in maize (Zea mays L.). MicroRNAs (miRNAs) are known to play critical roles in regulation of plant growth, development, and adaptation to abiotic and biotic stresses. To elucidate the roles of miRNAs in the regulation of maize in response to RBSDV, we employed high-throughput sequencing technology to analyze the miRNAome and transcriptome following RBSDV infection. A total of 76 known miRNAs, 226 potential novel miRNAs and 351 target genes were identified. Our dataset showed that the expression patterns of 81 miRNAs changed dramatically in response to RBSDV infection. Transcriptome analysis showed that 453 genes were differentially expressed after RBSDV infection. GO, COG and KEGG analysis results demonstrated that genes involved with photosynthesis and metabolism were significantly enriched. In addition, twelve miRNA-mRNA interaction pairs were identified, and six of them were likely to play significant roles in maize response to RBSDV. This study provided valuable information for understanding the molecular mechanism of maize disease resistance, and could be useful in method development to protect maize against RBSDV.
作者：Li, Aiqin; Li, Guanghui; Zhao, Yuhan; 等
作者单位：山东省农业科学院
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.122
出版年份：2018
出版刊次：9
点击下载：结合小RNA和基因表达分析揭示了miRNAs在玉米响应水稻黑条矮缩病毒侵染中的作用