中文摘要：大多数植物病毒依赖介体昆虫进行传播。病毒侵染后，病毒衍生的小干扰RNA（vsiRNAs）可以靶向病毒和宿主转录。水稻条纹病毒（RSV）是由灰飞虱传播的一种持久、增殖型病毒，可导致严重的水稻病害。为了研究vsiRNAs如何调节宿主植物和昆虫载体中的基因表达，本文分析了RSV感染的水稻和RSV感染的稻飞虱中小RNA（sRNA）和mRNA的表达谱。研究结果表明，同一病毒感染后在不同寄主中产生不同的vsiRNAs，从而可能会调节不同的生物进程，反映出不同的病毒-宿主相互作用。
外文摘要：Background: Most plant viruses depend on vector insects for transmission. Upon viral infection, virus-derived small interfering RNAs (vsiRNAs) can target both viral and host transcripts. Rice stripe virus (RSV) is a persistent-propagative virus transmitted by the small brown planthopper (Laodelphax striatellus, Fallen) and can cause a severe disease on rice. Results: To investigate how vsiRNAs regulate gene expressions in the host plant and the insect vector, we analyzed the expression profiles of small RNAs (sRNAs) and mRNAs in RSV-infected rice and RSV-infected planthopper. We obtained 88,247 vsiRNAs in rice that were predominantly derived from the terminal regions of the RSV RNA segments, and 351,655 vsiRNAs in planthopper that displayed relatively even distributions on RSV RNA segments. 38,112 and 80,698 unique vsiRNAs were found only in rice and planthopper, respectively, while 14,006 unique vsiRNAs were found in both of them. Compared to mock-inoculated rice, 273 genes were significantly down-regulated genes (DRGs) in RSV-infected rice, among which 192 (70.3%) were potential targets of vsiRNAs based on sequence complementarity. Gene ontology (GO) analysis revealed that these 192 DRGs were enriched in genes involved in kinase activity, carbohydrate binding and protein binding. Similarly, 265 DRGs were identified in RSV-infected planthoppers, among which 126 (47.5%) were potential targets of vsiRNAs. These planthopper target genes were enriched in genes that are involved in structural constituent of cuticle, serine-type endopeptidase activity, and oxidoreductase activity. Conclusions: Taken together, our results reveal that infection by the same virus can generate distinct vsiRNAs in different hosts to potentially regulate different biological processes, thus reflecting distinct virus-host interactions.
外文关键词：Rice; Rice stripe virus; Small brown planthopper; Virus-derived small interfering RNAs; Small RNA sequencing; Transcriptome
作者：Yang, Meiling; Xu, Zhongtian; Zhao, Wan; 等
作者单位：中国科学院
期刊名称：BMC PLANT BIOLOGY
期刊影响因子：3.93
出版年份：2018
出版刊次：10
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