中文摘要：凝集素类受体激酶（LecRLKs）是响应植物活体营养型病原体的关键因子。本研究旨在系统分析番茄LecRLKs对番茄响应番茄黄化曲叶病毒（TYLCV）侵染的影响。使用全基因组分析共鉴定了93个番茄SlyLecRLK基因，包括69个G型LecRLKs，23个L型LecRLKs和一个C型LecRLK，并使用系统发育分析将其聚类成3个亚科。转录组学分析显示大多数SlyLecRLK基因受植物激素和TYLCV侵染的调节。基因本体富集分析显示所有SlyLecRLK基因都和受体蛋白激酶活性及蛋白质修饰有关。研究结果表明，SlyLecRLK基因是TYLCV抗性的关键因子，也为揭示番茄SlyLecRLKs抵抗TYLCV感染的保护作用提供了重要信息。
外文摘要：Lectin receptor-like kinases (LecRLKs) are crucial factors in response to plant biotrophic pathogens. This study aimed to systemically analyze the effect of tomato LecRLKs on the responses to tomato yellow leaf curl virus (TYLCV) infection. A total of 93 tomato SlyLecRLK genes, including 69 G-type LecRLKs, 23 L-type LecRLKs, and one C-type LecRLK, were identified using genome-wide analysis and were clustered into three subfamilies using phylogenetic analysis. These SlyLecRLK genes were localized on the 12 tomato chromosomes with some gene duplication events. Transcriptomic analysis showed most of SlyLecRLKs were modulated by phytohormones and TYLCV infection. Gene ontology (GO) enrichment analysis showed all SlyLecRLK genes were associated with receptor protein kinase activity and protein modification. Quantitative RT-PCR (qRT-PCR) and gene interference validation showed SlyLecRLKs007, SlyLecRLKs036, and SlyLecRLKs044 genes were inhibited by TYLCV infection, and silencing of them were accompanied by accumulation of TYLCV DNA content. Subcellular localization of LecRLKs023 and LecRLKs044 on cell membranes was determined using a SlyLecRLK-containing A. tumefaciens strain GV3101. These results showed SlyLecRLK genes were key factors for TYLCV resistance, and also provided crucial information uncovering the protective effects of SlyLecRLKs on resisting TYLCV infection in tomato.
外文关键词：Genome-wide analysis; Tomato; TYLCV; Lectin receptor-like kinases
作者：Zhao, Tongmin; Wang, Jinyan; Zhang, Baolong; 等
作者单位：南京农业大学
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER
期刊影响因子：1.844
出版年份：2018
出版刊次：6
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