中文摘要：高通量深度测序和变异检测表明，从灰飞虱传播的水稻植株和本氏烟草植株上获得的水稻条纹病毒（RSV）种群的变异是单核苷酸多态性（SNPs）和插入缺失（InDels）。SNPs在RSV基因组中更均匀，但InDels主要发生在基因间区域（IRs）和5'或3'非编码区域。虽然插入的序列全部来自病毒本身，但没有明确的InDels模式。在RdRP ORF、IR 和运动蛋白ORF中分别观察到有6个、1个、1个非同义取代。这些非同义取代是稳定的，可导致NBL11 RSV种群中新的一致序列。此外，来自本氏烟草植株的RSV基因组中的SNPs和InDels远远高于来自O. sativa植株的数量。这些差异可能是由不同寄主植物产生的选择压力引起的。
外文摘要：High-throughput deep sequencing and variant detection showed that variations of Rice stripe virus (RSV) populations obtained from small brown planthopper-transmitted rice plants and sap-inoculated N. benthamiana plants were single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels). The SNPs were more uniform across RSV genome, but InDels occurred mainly in the intergenic regions (IRs) and in the 5' or 3' noncoding regions. There were no clear patterns of InDels, although the inserted sequences were all from virus itself. Six, one, and one non-synonymous substitutions were respectively observed in the RdRP ORF, IR and the movement protein ORF. These non-synonymous substitutions were found to be stable, resulting in new consensus sequences in the NBL11 RSV population. Furthermore, the numbers of SNPs and InDels in RSV genome from N. benthamiana plants were much higher than that from O. sativa plants. These differences are likely caused by selection pressures generated by different host plants.
外文关键词：Rice stripe virus; High-throughput deep sequencing; Population structure; Genetic variation; O. sativa; N. benthamiana
作者：Lu, Lina; Wu, Sanling; Jiang, Jun; 等
作者单位：浙江大学
期刊名称：VIROLOGY
期刊影响因子：3.374
出版年份：2018
出版刊次：11
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