中文摘要：水稻黑条矮缩病毒（RBSDVD），由灰飞虱传播，可引起水稻严重减产。培育抗性品种是控制病毒病害及其介体最为有效的策略之一。通过田间自然接种和温室中改良育苗箱筛选试验发现，籼稻品种WR24对RBSDVD和SBPH具有高抗性。利用来自WR24和易感粳稻Suyunuo杂交的153个品系组成的F-2：3种群进行RBSDVD和SBPH抗性的数量性状基因座（QTL）分析。构建由130个SSR标记组成的连锁图谱，平均标记间隔为13.90cM，总共1890.9cM。研究发现，所有抗性等位基因均来自WR24。在这些QTL中，qRBSDV7(WR24)、qSBPH5(WR24)和qSBPH10(WR24)是最新报道，并且qSBPH10(WR24)显示出主要的效应为释放了总表型变异的17.9%。RBSDVD和SBPH抗性QTLs和紧密连锁的DNA标记可用于水稻的RBSDV和SBPH抗性育种。
外文摘要：Rice black-streaked dwarf virus disease (RBSDVD), transmitted by small brown planthopper (SBPH, Laodelphax striatellus), causes serious loss in rice production. Breeding resistant cultivars are one of the most effective strategies to control the virus disease and its vector. By both natural inoculations in the field and modified seedling-box screening test in the glasshouse, an indica variety WR24 showed high resistance to RBSDVD and SBPH. An F-2:3 population consisting of 153 lines derived from a cross between WR24 and a susceptible japonica variety Suyunuo was used for quantitative trait loci (QTL) analysis of RBSDVD and SBPH resistance. The linkage map consisting of 130 SSR markers was constructed with an average marker interval of 13.90 cM, spanning a total of 1890.9 cM. Totally, five QTLs for RBSDV resistance, viz. qRBSDV3(WR24), qRBSDV6(WR24), qRBSDV7(WR24), qRBSDV9(WR24) and qRBSDV11(WR24), were detected on chromosomes 3, 6, 7, 9 and 11, with LOD scores of 2.7, 3.08, 3.13, 5.28 and 3.7, respectively. Meanwhile, three QTLs for SBPH resistance, including qSBPH5(WR24), qSBPH7(WR24) and qSBPH10(WR24), were mapped on chromosomes 5, 7 and 10, with LOD scores of 2.18, 3.5 and 3.57, respectively. All resistant alleles were from WR24. Among these QTLs, qRBSDV7(WR24), qSBPH5(WR24) and qSBPH10(WR24) were newly reported, and qSBPH10(WR24) showed major effect that explained 17.9% of total phenotypic variance. The RBSDVD and SBPH resistance QTLs and the tightly linked DNA markers can be utilized in RBSDV and SBPH resistance breeding in rice.
外文关键词：quantitative trait loci; rice (Oryza sativa L.); rice black-streaked dwarf virus disease; small brown planthopper
作者：Xu, Tingting; Liu, Yuqiang; Zhang, Le; 等
作者单位：南京农业大学
期刊名称：PLANT BREEDING
期刊影响因子：1.392
出版年份：2018
出版刊次：10
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