中文摘要：自2008/2009年以来，在阿根廷作物产区水稻植株上发现了病毒侵染的症状。本研究主要目的在于确定水稻植株皱缩的致病因子。因此，我们通过血清学、电子显微镜和RT-PCR方法分析了来自柏林de Astrada和San Javier两个省的有症状水稻植株样品，确定了水稻条纹坏死病毒（RSNV）的存在。这不仅是阿根廷发生RSNV的首次报道，也是该国水稻植株感染病毒的首次报道。
外文摘要：Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed samples of symptomatic rice plants from Berlin de Astrada (Corrientes province) and San Javier (Santa Fe province) by serological, electron microscopy and RT-PCR methods, and determined the presence of Rice stripe necrosis virus (RSNV). Helically constructed rod-shaped virus particles, with an axial canal with an estimated modal length of 228 nm, 370 nm and a last interval of 87-181 rim and a diameter of 20 nm, were observed in ultrathin sections and ISEM-D from rice leaf samples. Transmission of RSNV by viruliferous soil was achieved. Phylogenetic analysis, based on a partial sequence (828 nt) of the helicase gene of 10 RSNV isolates, grouped the Argentinian sequences in the same Glade as the African, rather than with the sequence from Colombia. This is the first report not only of the presence of RSNV in Argentina, but of a viral infection in rice plants in the country.
外文关键词：Oryza sativa; Benyvirus; RSNV; Plant virus
作者：Fernanda Maurino, Maria; Gimenez Pecci, Maria de la Paz; Daniel Kruger, Raul; 等
作者单位：Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
期刊名称：CROP PROTECTION
期刊影响因子：1.92
出版年份：2018
出版刊次：12
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