中文摘要：Tospovirus是一种tripartite负链RNA病毒，被认为是最具破坏性的植物病毒之一。病毒成功侵染植株需要许多宿主因子。迄今为止，已经鉴定出在Tospovirus侵染植株中具有重要作用的宿主因子非常少。之前已报道过，由番茄斑萎病毒（TSWV）编码的NSm蛋白是正番茄斑萎病毒属，在病毒细胞间和长距离运动中起着关键作用。本研究确定分子伴侣NbSGT1与本塞姆氏烟草中的TSWV NSm相互作用。本研究结果和先前公布的结果表明，分子伴侣NbSGT1在调节正链和tripartite负链RNA病毒侵染植物中起着重要作用。
外文摘要：Tospovirus is a tripartite negative stranded RNA virus and is considered as one of the most devastating plant viruses. Successful virus infection in plant requires many host factors. To date, very few host factors have been identified as important in Tospovirus infection in plants. We reported earlier that NSm protein encoded by Tomato spotted wilt virus (TSWV), a type species of the genus Orthotospovirus, plays critical roles in viral cell-to-cell and long-distance movement. In this study, we determined that molecular co-chaperone NbSGT1 interacted with TSWV NSm in Nicotiana benthamiana. TSWV infection significantly upregulated the expression of NbSGT1 gene and transient overexpression of NbSGT1 in N.benthamiana leaves accelerated TSWV infection. In contrast, silencing the NbSGT1 gene expression using a virus-induced gene silencing (VIGS) approach strongly inhibited TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infection in N. benthamiana plants. Furthermore, NbSGT1 was found to regulate the infection of both American and Euro/Asia type tospoviruses in N. benthamiana plant. Collectively, our findings presented in this paper and the results published previously indicated that molecular co-chaperone NbSGT1 plays important roles in modulating both positive stranded and tripartite negative stranded RNA virus infection in plants.
外文关键词：molecular co-chaperone; SGT1; Tomato spotted wilt virus; TSWV NSm; tripartite negative stranded RNA virus
作者：Qian, Xin; Xiang, Qing; Yang, Tongqing; 等
作者单位：南京农业大学
期刊名称：VIRUSES-BASEL
期刊影响因子：3.811
出版年份：2018
出版刊次：11
点击下载：分子伴侣蛋白SGT1对本塞姆氏烟草中番茄斑萎病毒的细胞间移动和系统感染至关重要