中文摘要：南方水稻黑条矮缩病毒（SRBSDV）和水稻粗糙特技病毒（RRSV）都属于呼肠孤病毒科，这两种病毒的协同感染通常发生在野外。该研究表明，SRBSDV和RRSV均影响RNA干扰（RNAi）途径，并在水稻中形成不同的病毒衍生的干扰RNA（vsiRNA）图谱。SRBSDV和RRSV共同感染水稻上调了水稻DCL蛋白的表达，但下调了水稻RDRs的表达，并且相对于单独感染来说，不管是RBSDV还是RRSV的vsiRNA的积累都减少了。无论植物是单独感染SRBSDV还是与RRSV共感染，大多数SRBSDV vsiRNA的长度为21nt或22nt。此外，大多数RRSV vsiRNA的长度为20 nt、21 nt或22 nt，其中20 nt长度占最大比例；与SRBSDV共感染进一步增加了20nt vsiRNA的比例，并降低了21nt vsiRNA的比例。共感染对vsiRNA的链拍好性和热点没有影响，但显著改变了5'末端核苷酸的偏好性。本研究为进一步研究SRBSDV和RRSV的发病机制和协同机制提供了参考。
外文摘要：Both Southern rice black-streaked dwarf virus (SRBSDV) and Rice ragged stunt virus (RRSV) belong to the family Reoviridae, and synergistic infection of these two viruses commonly occurs in the field. This study revealed that both SRBSDV and RRSV affect the RNA interference (RNAi) pathway and form different virus-derived interfering RNA (vsiRNA) profiles in rice. Co-infection of rice by SRBSDV and RRSV up-regulated the expression of rice DICER-like (DCL) proteins but down-regulated the expression of rice RNA-dependent RNA polymerases (RDRs), and the accumulation of vsiRNAs of either RBSDV or RRSV was decreased compared with that in singly infected plants. The majority of SRBSDV vsiRNAs were 21 nt or 22 nt in length, whether plants were singly infected with SRBSDV or co-infected with RRSV. On the other hand, the majority of RRSV vsiRNAs were 20 nt, 21 nt, or 22 nt in length, among which those 20 nt in length accounted for the largest proportion; co-infection with SRBSDV further increased the proportion of 20 nt vsiRNAs and decreased the proportion of 21 nt vsiRNAs. Co-infection had no effects on the strand favoritism and hot spots of the vsiRNAs, but changed the bias of the 5' terminal nucleotide significantly. This study provides a reference for further study on the pathogenesis and synergistic mechanism of SRBSDV and RRSV.
外文关键词：southern rice black-streaked dwarf virus (SRBSDV); rice ragged stunt virus (RRSV); synergism; RNA interference (RNAi); virus-derived interfering RNAs (vsiRNAs)
作者：Li, Zhanbiao; Zhang, Tong; Huang, Xiuqin; 等
作者单位：华南农业大学
期刊名称：VIRUSES-BASEL
期刊影响因子：3.811
出版年份：2018
出版刊次：11
点击下载：两种呼肠孤病毒及其协同感染对水稻 RNAi系统和vsiRNA生产的影响