中文摘要：由叶蝉传播的水稻条纹花叶病毒（RSMV）是最近在华南地区鉴定的细胞质弹状病毒属的一个新物种。为了探索其地理分布和遗传多样性，本研究进行了野外调查和病毒全基因组测序。结果表明，RSMV存在于中国南方采集的水稻样品中。选择来自不同地理区域的12个代表性样品用于病毒全基因组测序，并且结合先前报道的RSMV分离物进行病毒基因组变异分析。同源性分析表明，13个RSMV分离株的基因组序列与核苷酸同一性高度一致。基于RSMV全基因组核苷酸序列的系统发育树揭示了所有RSMV分离株可基于地理起源分成两组。广西和海南分离株的L蛋白分别具有5个和1个特定氨基酸位点，这表明L基因在RSMV分散过程中经历了环境适应性变异。
外文摘要：Rice stripe mosaic virus (RSMV) transmitted by the leafhopper Recilia dorsalis is a tentative new species in the genus Cytorhabdovirus identified recently in South China. To explore its geographic distribution and genetic diversity, field investigation and viral whole-genome sequencing were conducted in this study. The results indicated that RSMV was present in the rice samples collected across southern China. Twelve representative samples from different geographical regions were selected for viral whole-genome sequencing and the viral genome variation was analyzed in combination with a previously reported RSMV isolate. Identity analysis showed that the genome sequences of 13 RSMV isolates were highly conserved with nucleotide identities over 99.4%. There was a strong negative selection pressure during the evolution of RSMV with more transitions (72.08%) than transversions (27.92%) found between the RSMV isolates. Among the seven genes encoded by RSMV, the P gene was the most variable, followed by N, M, L, and G; the P3 and P6 amino acid sequences were not found to be mutated and no mutations were found in the non-coding region. A phylogenetic tree based on the RSMV whole-genome nucleotide sequences revealed that all RSMV isolates clustered in two groups based on geographical origin. Notably, the L proteins of the Guangxi and Hainan isolates had five and one specific amino acid sites, respectively, suggesting that the L gene has undergone environmental adaptive variation during the dispersal of RSMV.
外文关键词：rice stripe mosaic virus; Cytorhabdovirus; virus genetic diversity; rice viral disease; leafhopper-transmitted virus
作者：Yang, Xin; Chen, Biao; Zhang, Tong; 等
作者单位：华南农业大学
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.259
出版年份：2018
出版刊次：12
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