中文摘要：水稻黑条矮缩病毒（RBSDV）可感染主要作物水稻，该病毒通过灰飞虱（SBPH：Laodelphax striatellus Fallen）传播，在中国造成了重大经济损失。为了快速诊断RBSDV，使用P10病毒特异性引物和探针开发了逆转录-重组酶聚合酶扩增（RT-RPA）方法。 用横向流动试纸条技术实现末端标记扩增产物的检测。 研究结果表明，RT-RPA和RT-PCR测定在使用cDNA作为模板的RBSDV检测中表现出了相似的灵敏度和特异性。最佳RT-RPA反应温度和时间分别为37℃和20分钟。 通过筛选21个野外疑似水稻植株，证实RT-RPA测定法简单、快速和可靠。 因此，本研究开发的RBSDV RT-RPA测定法将是成功快速诊断RBSDV感染水稻植株的一种工具。
外文摘要：Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallen), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method was developed using P10 virus-specific primers and probes. Detection of terminally labeled amplification products was achieved with the lateral flow strip method. Our results demonstrate that RT-RPA and RT-PCR assays offer similar sensitivity and specificity in RBSDV detection using cDNA as template. The optimum RT-RPA reaction temperature and time was 37 degrees C and 20 min, respectively. By screening twenty-one field suspected rice plants, the RT-RPA assay was confirmed to be simple, rapid and reliable. Thus, the RBSDV RT-RPA assay developed here will be a successful tool for quick diagnosis of RBSDV-infected rice plants.
外文关键词：RBSDV; Recombinase polymerase amplification; Lateral flow strip
作者：Zhao, Chong; Sun, Feng; Li, Xuejuan; 等
作者单位：南京农业大学
期刊名称：JOURNAL OF VIROLOGICAL METHODS
期刊影响因子：1.746
出版年份：2019
出版刊次：1
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