中文摘要：由于真菌、细菌、病毒和昆虫的生物胁迫导致的产量损失是大多数水稻种植区域的一个重要挑战。开发耐药栽培品种被认为是减少水稻减产的一种有效且可持续的方法。本研究描述了带有多种抗性基因的粳稻基因渗入系(MR系，对四种不同类型的生物胁迫均具有抗性)的发育，并将这些品系的农艺性状、产量和谷粒品质参数与其回交亲本进行对比。通过分子标记辅助回交法共开发了9个MR系，所有MR系都含有共同的R基因Bph18和qSTV11（SG）以及两个Pi基因（Pib + Pik），分别对褐飞虱（BPH）、水稻条纹病毒（RSV）和稻瘟病具有抗性。
外文摘要：Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11(SG) and two Pi genes (Pib+Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18+qSTV11SG+Pib+Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.
外文关键词：Multiple resistance genes; Biotic stress; Gene pyramiding; Japonica rice; Marker-assisted selection
作者：Reinke, Russell; Kim, Suk-Man; Kim, Bo-Kyeong
作者单位：国际水稻研究所
期刊名称：MOLECULAR GENETICS AND GENOMICS
期刊影响因子：2.879
出版年份：2018
出版刊次：12
点击下载：利用分子育种技术开发具有抗褐飞虱、白叶枯病、稻瘟病和水稻条纹病毒多抗基因的粳稻基因渗入系