中文摘要：双生病毒是重要的作物病毒病病原体，由烟粉虱物种复合体传播。虽然白粉虱传播双生病毒已被研究多年，但不同种类的烟粉虱复合体对不同病毒差异传播的调节机制仍然很大程度上未知。本研究首先比较了四种烟粉虱复合体对烟草曲茎病毒（TbCSV）的传播效率，发现亚洲II 1传播这种病毒的效率最高，而MEAM1的传播效率最低。其次，通过病毒定量分析和免疫荧光检测，发现亚洲II 1中TbCSV穿过中肠壁的效率高于MEAM1。最后，通过注射将亚洲II 1和MEAM1中血淋巴中的病毒粒子数量设定为相同水平，然后比较它们传播TbCSV的能力，结果发现它们传播TbCSV的差异变小了。总之，本研究结果表明，双生病毒穿过粉虱中肠壁到达载体血淋巴的效率在确定病毒传播方面起着重要作用。
外文摘要：Begomoviruses are important crop viral disease agents, and they are transmitted by whiteflies of the Bemisia tabaci complex. Although the transmission of begomoviruses by whiteflies has been studied for many years, the mechanisms governing differential transmission of begomoviruses by different species of the Bemisia tabaci complex remain largely unknown. Here we firstly compared the transmission efficiency of tobacco curly shoot virus (TbCSV) by four species of the B. tabaci complex and found that Asia II 1 transmitted this virus with the highest efficiency, whereas MEAM1 transmitted it with the lowest. Next, by performing quantitative analysis of virus and immune-fluorescence detection, we found that the efficiency of TbCSV to cross the midgut wall was higher in Asia II 1 than in MEAM1. Finally, we set the quantities of virions in the haemolymph to the same level in Asia II 1 and MEAM1 via injection and then compared their capacity in TbCSV transmission, and found that the difference in TbCSV transmission between them became smaller. Taken together, our findings suggest that the efficiency of a begomovirus to cross the midgut wall of a whitefly to reach the vector's haemolymph plays a significant role in determining transmission of the virus.
外文关键词：begomovirus; Bemisia tabaci; differential transmission; midgut
作者：Pan, Lilong; Chen, Qunfang; Guo, Tao; 等
作者单位：浙江大学
期刊名称：SCIENCE CHINA-LIFE SCIENCES
期刊影响因子：3.583
出版年份：2018
出版刊次：10
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