中文摘要：佛手瓜是一种单籽葫芦科蔬菜作物。2015至2016年间，在泰米尔纳德邦丘陵地区的佛手瓜植株中观察到斑驳和叶片扭曲类型的症状。丁迪古尔地区该病害发病率为为50.3-100％，产量损失约为69.9％。受感染的佛手瓜植株呈黄色斑点、黄色斑驳、叶片卷曲、褶皱。被感染植株的果实畸形，无法销售。引起该病的菜豆金色花叶病毒属被鉴定为是新德里番茄曲叶病毒的一种变体。佛手瓜ToLCNDV分离株与其他ToLCNDV分离株仅有91％至92％的同一性，因此被认定为不同的菌株。本研究是关于ToLCNDV种子传播性质的首次报道，并讨论了其在跨越几个国家的病毒越境转移中的意义。
外文摘要：Chayote (Sechium edule (Jacq. Sw.) is a single seeded cucurbitaceous vegetable crop mainly grown for its fruit. During 2015-2016, mosaic and leaf distortion type of symptoms were observed in chayote plants in hilly regions of Tamil Nadu. The disease incidence was 50. 3-100% and yield loss was about 69.9% in Dindigul district. The infected chayote plants showed yellow spots, yellow mosaic, leaf curling, puckering, and enations. The fruits of infected plants were malformed and were not marketable. The begomovirus causing the disease was identified as a variant of tomato leaf curl New Delhi virus. The chayote isolates of ToLCNDV share only 91 to 92% identity with other ToLCNDV isolates, deserving to be designated as distinct strain. The phylogenetic analysis on the basis of DNA A component nucleotides clearly indicated common origin of chayote, ridge gourd, ash gourd isolates of India along with Spanish isolates of ToLCNDV. This was contrasting to diverse origin of ToLCNDV isolates from other countries. The virus was sap transmissible to selected cucurbitaceous hosts. The whitefly population (Asia-I) reared in the glass house transmitted the virus to bottle gourd with 4 h of acquisition access period (AAP) and 24 h of inoculation feeding period (IFP). Heavy infestation of greenhouse whiteflies, Trialeurodes vaporariorum on infected chayote plant in Kodaikanal paved way to investigations on its role in vector transmission of ToLCNDV. The field population of T. vaporariorum was found to be viruliferous in PCR using virus specific primers. The greenhouse whitefly efficiently transmitted the virus with 4 h of AAP and 24 h IFP. Seed-borne nature of ToLCNDV was confirmed in PCR by using Roja's and ToLCNDV specific primers in different parts of the fruit viz., pericarp, mesocarp, seed coat, endosperm and embryo. This is the first report of seed transmissible nature of ToLCNDV, its implication in transboundary movement of the virus across several countries is discussed.
外文关键词：Chayote; Bemisia tabaci; Trialeurodes vaporariorum; Seed transmission
作者：Sangeetha, B.; Malathi, V. G.; Alice, D.; 等
作者单位：Tamil Nadu Agricultural University
期刊名称：VIRUS RESEARCH
期刊影响因子：2.736
出版年份：2018
出版刊次：10
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