中文摘要：最具经济重要性的植物病毒是由植食性昆虫特异性传播的，可显著影响病毒流行病学。大麦黄条点花叶病毒（BYSMV）是质型杆状病毒属的成员，由灰飞虱（SBPH， Laodelphax striatellus）以持续繁殖方式传播。然而，BYSMV在SBPHs中的感染途径知之甚少。在本研究中，开展了免疫荧光共聚焦激光扫描显微镜（iCLSM）检测以研究BYSMV在SBPH中的途径。结果意外地发现，BYSMV最初感染了SBPH的后肠上皮，而不是最初被其他持续性繁殖病毒感染的中肠上皮。随后，BYSMV向后肠内脏肌肉传播，并扩散到消化道、血淋巴和唾液腺的其他部位。通过不同的分子检测和免疫组织化学对病毒mRNA和BYSMV核蛋白基因表达的比较分析进一步证明BYSMV最初在SBPH的后肠上皮细胞中感染和复制。总的来说，本研究首次提出后肠是BYSMV初始感染的部位，这代表着发现了持续繁殖型病毒的一个新的传播途径。
外文摘要：The most economically important plant viruses are specifically transmitted by phytophagous insects that significantly affect viral epidemiology. Barley yellow striate mosaic virus (BYSMV), a member of the genus Cytorhabdovirus, is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus) in a persistent-propagative manner. However, the infection route of BYSMV in SBPHs is poorly understood. In this study, immunofluorescence confocal laser scanning microscopy (iCLSM) was performed to investigate the route of BYSMV in SBPHs. We unexpectedly found that BYSMV initially infected the hindgut epithelium of SBPHs, instead of the midgut epithelium initially infected by other persistent-propagative viruses. Subsequently, BYSMV disseminated to the hindgut visceral muscles and spread to other parts of alimentary canals, hemolymph, and salivary glands. Comparative analysis of gene expression on viral mRNAs and the BYSMV nucleoprotein by using different molecular detection and immunohistochemistry further demonstrated that BYSMV initially infected and replicated in the hindgut epithelial cells of SBPHs. Collectively, our study provides the first insight into that hindgut is initial infection site of BYSMV that represents a new dissemination route of persistent-propagative viruses.
外文关键词：Barley yellow striate mosaic virus; cytorhabdovirus; persistent-propagative virus; Laodelphax striatellus; hindgut
作者：Cao, Qing; Xu, Wen-Ya; Gao, Qiang; 等
作者单位：中国农业大学
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.259
出版年份：2018
出版刊次：6
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