中文摘要：大多数取食韧皮部的昆虫会在取食过程中分泌蜜露及唾液。但是，目前对唾液蛋白的功能仍知之甚少。本研究目的在于揭示水稻刺吸式害虫褐飞虱的唾液成分与功能。完成褐飞虱整个基因组和转录组的测序，有助于阐明唾液蛋白质的遗传信息与表达特异性。本研究结果将非常有助于理清褐飞虱生理过程中唾液蛋白的具体功能，并阐明褐飞虱与水稻之间的交互机制，为水稻虫害的管理提供了重要的目标。
外文摘要：Most phloem-feeding insects secrete gelling and watery saliva during the feeding process. However, the functions of salivary proteins are poorly understood. In this study, our purpose was to reveal the components and functions of saliva in a rice sap-sucking insect pest, Nilaparvata lugens. The accomplishment of the whole genome and transcriptome sequencing in N. lugens would be helpful for elucidating the gene information and expression specificity of the salivary proteins. In this study, we have, for the first time, identified the abundant protein components from gelling and watery saliva in a monophagous sap-sucking insect species through shotgun proteomic detection combined with the genomic and transcriptomic analysis. Eight unknown secreted proteins were limited to N. lugens, indicating species-specific saliva components. A group of annexin-like proteins first identified in the secreted saliva displayed different domain structure and expression specificity with typical insect annexins. Nineteen genes encoding five annexin-like proteins, six salivaps (salivary glands-specific proteins with unknown function), seven putative enzymes, and a mucin-like protein showed salivary gland specific expression pattern, suggesting their importance in the physiological mechanisms of salivary gland and saliva in this insect species. RNA interference revealed that salivap-3 is a key protein factor in forming the salivary sheath, while annexin-like5 and carbonic anhydrase are indispensable for N. lugens survival. These novel findings will greatly help to clarify the detailed functions of salivary proteins in the physiological process of N. lugens and elucidate the interaction mechanisms between N. lugens and the rice plant, which could- provide important targets for the future management of rice pests.
外文关键词：planthopper；salivary protein；proteome；salivap；annexin-like；CA；RNA interference
作者：Huang, Hai-Jian；Liu, Cheng-Wen；Huang, Xiao-Hui；等
作者单位：浙江大学
期刊名称：JOURNAL OF PROTEOME RESEARCH
期刊影响因子：4.173
出版年份：2016
出版刊次：6
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