中文摘要：茉莉酸及其相关代谢产物在植物防御和生长中发挥了重要作用。茉莉酸羧基甲基转移酶（JMT）参与了植物的防御与生长，可将茉莉酸（JA）甲基化成茉莉酸甲酯（MeJA），从而影响了茉莉酸（JA）及其相关代谢产物的浓度。然而，尚未有很好的关于单子叶植物防御与生长中茉莉酸羧基甲基转移酶（JMT）基因的分子水平特征的研究。本研究克隆了水稻JMT 基因 OsJMT1（其编码蛋白位于细胞质基质中），发现重组OsJMT1蛋白可催化JA生成MeJA。当水稻遭到褐飞虱侵染时，OsJMT1的表达会上调。本研究结果表明，OsJMT1可改变JA和相关代谢物的水平，在植物生长和植食性昆虫诱导的防御反应调节中起着重要的作用。
外文摘要：Jasmonic acid (JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase (JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate (MeJA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene, OsJMT1, whose encoding protein was localized in the cytosol, we found that the recombinant OsJMT1 protein catalyzed JA to MeJA. OsJMT1 is up-regulated in response to infestation with the brown planthopper (BPH; Nilaparvata lugens). Plants in which OsJMT1 had been overexpressed (oe-JMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased MeJA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine (JA-Ile). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs, probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H2O2 and MeJA in oe-JMT plants. These results indicate that OsJMT1, by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice.
外文关键词：Herbivore-induced plant defense；jasmonic acid；jasmonic acid carboxyl methyltransferase；jasmonoyl-isoleucine；methyl jasmonate；Nilaparvata lugens；rice
作者：Qi, Jinfeng；Li, Jiancai；Han, Xiu；等
作者单位：浙江大学
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY
期刊影响因子：3.67
出版年份：2016
出版刊次：6
点击下载：茉莉酸羧基甲基转移酶可调节水稻的发育及植食性昆虫诱导的防御反应