中文摘要：为提高南方优良籼稻品种Hemeizhan (HMZ)的褐飞虱抗性，采用分子标记辅助回交法（MABC）将三种BPH抗性基因（Bph3、Bph14和Bph15）一起导入到 HMZ的遗传背景中。在第三代回交后代中获得了轮回亲本基因型恢复率分别达到96%以上、89%以上的近等基因系（Bph3-NIL、Bph14-NIL、 Bph15-NIL 和 Bph14+Bph15-NIL）及基因聚合系（Bph3+Bph14-PYL、 Bph3+Bph15-PYL 和 Bph3+Bph14+Bph15-PYL），在幼苗期与孕穗期的BPH抗性高于HMZ。本研究得到的这些改良系为其他籼稻品种广谱、持久的BPH抗性的提高提供了很好的中间来源。本研究结果表明，MABC是提高优良籼稻品种BPH抗性的有效渠道。
外文摘要：To improve brown planthopper (Nilaparvata lugens Stal; BPH) resistance of an elite indica cultivar of South China, Hemeizhan (HMZ), we applied marker-assisted backcross (MABC) to incorporate three BPH-resistance genes (Bph3, Bph14 and Bph15) into the genetic background of HMZ. In the third backcross (BC3) generation, we obtained near-isogenic lines (Bph3-NIL, Bph14-NIL, Bph15-NIL and Bph14+Bph15-NIL) with more than 96% recovery of recurrent parent genome, and pyramided lines (Bph3+Bph14-PYL, Bph3+Bph15-PYL and Bph3+Bph14+Bph15-PYL) with more than 89% recovery of recurrent parent genome. These lines showed stronger resistance against BPH than HMZ at seedling and booting stages. The rank of resistance gene effect was Bph3+Bph14+Bph15Bph3+Bph15Bph3+Bph14Bph14+Bph15Bph3Bph15Bph14>none. Compared with HMZ, only Bph3+Bph14+Bph15-PYL had a significant difference in yield per plant, and the lines carrying Bph3 had higher amylose contents, indicating that Bph3 was tightly linked to Wx(a) allele. These improved lines are good intermediate sources of broad-spectrum and durable BPH resistance to improve other indica cultivars. Our results demonstrate that MABC is a very efficient approach to improve BPH resistance of elite rice cultivar.
外文关键词：Oryza sativa L；brown planthopper-resistance gene；marker-assisted selection；near-isogenic lines；pyramided lines；agronomic trait
作者：Hu, Wei；Xiao, Hanxiang；Hu, Kan；等
作者单位：广东农业科学院
期刊名称：PLANT BREEDING
期刊影响因子：1.502
出版年份：2016
出版刊次：6
点击下载：应用分子标记辅助回交法将Bph3、Bph14 和Bph15导入到优良籼稻中以增强水稻对褐飞虱的抗性