中文摘要：褐飞虱是水稻种植区最具破坏性的害虫。褐飞虱抗性等位基因的信息可用性及其来源促进了BPH抗性育种进程。本研究通过基因特异性标记筛选出了260个高度多样化水稻品种或选育系（含有5种主要的BPH抗性基因Bph10、Bph13、Bph18、Bph20和Bph21）。经分析发现，260个品种中有137个品种至少含有一种BPH抗性基因。本研究发现的BPH抗性品种可作为替代性抗性基因来源，也是一种新的BPH抗性基因资源。研究开发的Bph18标记将对今后BPH抗性品种的分子育种非常有用。
外文摘要：Brown planthopper (BPH) is the most devastating insect pest in rice-growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH-resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene-specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR-based co-dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH-resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH-resistant breeding programmes.
 
外文关键词：brown planthopper；resistance genes；molecular marker；genetic diversity；Bph18
作者：Ramkumar, Gandhimani；Prahalada, G. D.；Hechanova, Sherry Lou；等
作者单位：国际水稻研究所
期刊名称：PLANT BREEDING
期刊影响因子：1.502
出版年份：2016
出版刊次：6
点击下载：探索含有BPH抗性基因的水稻品种遗传多样性及Bph18SNP标记的发展