中文摘要：巨自噬/自噬在哺乳动物及植物抵抗病原菌的侵染中起着重要的作用。然而，对于自噬在昆虫介体与其传播的植物病毒交互中的作用仍知之甚少。菜豆金色花叶病毒是一种单链DNA病毒，仅由烟粉虱以持久的方式传播。本研究通过自噬体和ATG8-II的形成，证明了菜豆金色花叶病毒属番茄黄化曲叶病毒（TYLCV）的侵染可激活MEAM1烟粉虱的自噬途径，并发现激活烟粉虱自噬可以抑制病毒传播的效率，而抑制自噬则会有助于病毒的传播。本研究表明，TYLCV侵染可激活烟粉虱自噬途径，随后会引起病毒的降解。此外，本研究证明了昆虫介体可利用自噬抑制循环传播的植物病毒的侵染，且TYLCV可复制并引起病毒与昆虫介体之间复杂的交互作用。
外文摘要：Macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. However, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. Begomoviruses are a group of single-stranded DNA viruses and are exclusively transmitted by the whitefly Bemisia tabaci in a circulative manner. In this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (TYLCV) could activate the autophagy pathway in the Middle East Asia Minor 1 (MEAM1) species of the B. tabaci complex as evidenced by the formation of autophagosomes and ATG8-II. Interestingly, the activation of autophagy led to the subsequent degradation of TYLCV coat protein (CP) and genomic DNA. While feeding the whitefly with 2 autophagy inhibitors (3-methyladenine and bafilomycin A(1)) and silencing the expression of Atg3 and Atg9 increased the viral load; autophagy activation via feeding of rapamycin notably decreased the amount of viral CP and DNA in the whitefly. Furthermore, we found that activation of whitefly autophagy could inhibit the efficiency of virus transmission; whereas inhibiting autophagy facilitated virus transmission. Taken together, these results indicate that TYLCV infection can activate the whitefly autophagy pathway, which leads to the subsequent degradation of virus. Furthermore, our report proves that an insect vector uses autophagy as an intrinsic antiviral program to repress the infection of a circulative-transmitted plant virus. Our data also demonstrate that TYLCV may replicate and trigger complex interactions with the insect vector.
外文关键词：autophagy; begomovirus; Bemisia tabaci; interaction; Tomato yellow leaf curl virus; virus transmission; whitefly
作者：Wang, Lan-Lan；Wang, Xin-Ru；Wei, Xue-Mei；等
作者单位：浙江大学
期刊名称：AUTOPHAGY
期刊影响因子：9.108
出版年份：2016
出版刊次：9
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